The in vitro effect of different PRP concentrations on osteoblasts and fibroblasts

Graziani, Filippo, Ivanovski, Saso, Cei, Silvia, Ducci, Francesco, Tonetti, Maurizio and Gabriele, Mario (2006) The in vitro effect of different PRP concentrations on osteoblasts and fibroblasts. Clinical Oral Implants Research, 17 2: 212-219. doi:10.1111/j.1600-0501.2005.01203.x

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Author Graziani, Filippo
Ivanovski, Saso
Cei, Silvia
Ducci, Francesco
Tonetti, Maurizio
Gabriele, Mario
Title The in vitro effect of different PRP concentrations on osteoblasts and fibroblasts
Journal name Clinical Oral Implants Research   Check publisher's open access policy
ISSN 0905-7161
Publication date 2006
Sub-type Article (original research)
DOI 10.1111/j.1600-0501.2005.01203.x
Open Access Status File (Author Post-print)
Volume 17
Issue 2
Start page 212
End page 219
Total pages 8
Editor N. P. Lang
Place of publication Denmark
Publisher Blackwell Munksgaard
Collection year 2006
Language eng
Subject C1
320899 Dentistry not elsewhere classified
730112 Oro-dental and disorders
Abstract Objectives:The aim of this study was to assess the biological rationale for the use of platelet-rich plasma (PRP) by evaluating the effect of different concentrations of PRP on osteoblasts (OB) and fibroblasts (FB) function in vitro. Materials and methods:PRP was obtained from volunteer donors using standard protocols. Primary human cultures of oral FBs and OBs were exposed to both activated and non-activated plasma as well as various concentrations of PRP (2.5 x, 3.5 x and max (4.2-5.5 x)). Cell proliferation was evaluated after 24 and 72 h using an MTT proliferation assay. Production of osteocalcin (OCN), osteoprotegerin (OPG) and transforming growth factor beta 1 (TGF-beta 1) was evaluated in OB after 24 and 72 h. Statistical analysis was performed using one-way ANOVA. Results:PRP-stimulated cell proliferation in both OBs and FBs. The effect of different PRP concentrations on cell proliferation was most notable at 72 h. The maximum effect was achieved with a concentration of 2.5 x, with higher concentrations resulting in a reduction of cell proliferation. Upregulation of OCN levels and downregulation of OPG levels were noted with increasing PRP concentrations at both 24 and 72 h. TGF-beta 1 levels were stimulated by increasing concentrations of PRP, with the increased levels being maintained at 72 h. Conclusions:PRP preparations exert a dose-specific effect on oral FBs and OBs. Optimal results were observed at a platelet concentration of 2.5 x, which was approximately half of the maximal concentrate that could be obtained. Increased concentrations resulted in a reduction in proliferation and a suboptimal effect on OB function. Hence, different PRP concentrations may have an impact on the results that can be obtained in vivo.
Keyword Dentistry, Oral Surgery & Medicine
Engineering, Biomedical
Platelet-rich Plasma
Osteogenic Differentiation
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2007 Higher Education Research Data Collection
School of Dentistry Publications
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Citation counts: TR Web of Science Citation Count  Cited 156 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 187 times in Scopus Article | Citations
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Created: Wed, 15 Aug 2007, 08:14:52 EST