Prostaglandin E2 enhances transforming growth factor-beta 1 and TGF-beta receptors synthesis: An in vivo and in vitro study

Ramirez-Yanez, G. O., Hamlet, S., Jonarta, A., Seymour, G. J. and Symons, A. L. (2006) Prostaglandin E2 enhances transforming growth factor-beta 1 and TGF-beta receptors synthesis: An in vivo and in vitro study. Prostaglandins Leukotrienes and Essential Fatty Acids, 74 3: 183-192. doi:10.1016/j.plefa.2006.01.003


Author Ramirez-Yanez, G. O.
Hamlet, S.
Jonarta, A.
Seymour, G. J.
Symons, A. L.
Title Prostaglandin E2 enhances transforming growth factor-beta 1 and TGF-beta receptors synthesis: An in vivo and in vitro study
Formatted title
Prostaglandin E2 enhances transforming growth factor-beta 1 and TGF-beta receptors synthesis: An in vivo and in vitro study
Journal name Prostaglandins Leukotrienes and Essential Fatty Acids   Check publisher's open access policy
ISSN 0952-3278
Publication date 2006
Sub-type Article (original research)
DOI 10.1016/j.plefa.2006.01.003
Volume 74
Issue 3
Start page 183
End page 192
Total pages 10
Place of publication Edinburgh, U.K.
Publisher Churchill Livingstone
Collection year 2006
Language eng
Subject C1
320899 Dentistry not elsewhere classified
730112 Oro-dental and disorders
1105 Dentistry
Formatted abstract
The aims of this study were to determine how Prostaglandin E2 (PGE2) locally applied affected the immunodistribution of latent transforming growth factor-beta 1 (TGF-β1), and how the eicosanoid modified TGF-β1 release and TGF-β receptors gene expression in cultured osteoblasts. PGE2 locally delivered on the rat mandible at doses of 0.1 and 0.05 mg/day, but not 0.025 mg/day, over 20 days significantly increased latent TGF-β1 immunodistribution (P<0.001), comparing with a placebo-treated group. Cultured osteoblasts stimulated with 105 or 10−7 M PGE2 significantly varied the level of activated TGF-β1 released into supernatants at different experimental periods compared with negative and positive controls. TGF-β receptor type I gene expression was significantly increased in osteoblasts (P<0.01) after 10 days of treatment with 10−5 and 10−7 M PGE2, whereas 10−3 M PGE2 produced the opposite effect. It is concluded that PGE2 may stimulate bone deposition by affecting TGF-β pathway. This effect on the pathway appears to be dose-dependent.
Keyword Activation
Biochemistry
Bone
Cartilage
Cell biology
Differentiation
Endocrinology
Expression
Osteoblastic cells
Mechanism
Phenotype
Proteins
Rats
Molecular biology
Metabolism
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
2007 Higher Education Research Data Collection
School of Dentistry Publications
 
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Created: Wed, 15 Aug 2007, 08:13:38 EST