Measurement of action spectra of light-activated processes

Ross, Justin A., Zvyagin, Andrei V., Heckenberg, Norman R., Upcroft, Jacqui, Upcroft, Peter and Rubinsztein-Dunlop, Halina (2006) Measurement of action spectra of light-activated processes. Journal of Biomedical Optics, 11 1: 014008-1-014008-4. doi:10.1117/1.2161172

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Author Ross, Justin A.
Zvyagin, Andrei V.
Heckenberg, Norman R.
Upcroft, Jacqui
Upcroft, Peter
Rubinsztein-Dunlop, Halina
Title Measurement of action spectra of light-activated processes
Journal name Journal of Biomedical Optics   Check publisher's open access policy
ISSN 1083-3668
Publication date 2006
Sub-type Article (original research)
DOI 10.1117/1.2161172
Open Access Status File (Publisher version)
Volume 11
Issue 1
Start page 014008-1
End page 014008-4
Total pages 4
Editor Dr Bruce J. Tromberg
Karolyn Labes
Place of publication Bellingham, WA, United States
Publisher S P I E - International Society for Optical Engineering
Collection year 2006
Language eng
Abstract We report on a new experimental technique suitable for measurement of light-activated processes, such as fluorophore transport. The usefulness of this technique is derived from its capacity to decouple the imaging and activation processes, allowing fluorescent imaging of fluorophore transport at a convenient activation wavelength. We demonstrate the efficiency of this new technique in determination of the action spectrum of the light mediated transport of rhodamine 123 into the parasitic protozoan Giardia duodenalis. (c) 2006 Society of Photo-Optical Instrumentation Engineers.
Keyword Biochemical Research Methods
Radiology, Nuclear Medicine & Medical Imaging
Two-photon Microscopy
Giardia Duodenalis
Confocal Microscopy
Light-activated Transport
Confocial Microscopy
Q-Index Code C1

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Created: Wed, 15 Aug 2007, 08:10:40 EST