Identification of three gene candidates for multicellular resistance in colon carcinoma

Timmins, N. E., Maguire, T. L., Grimmond, S. M. and Nielsen, L. K. (2004) Identification of three gene candidates for multicellular resistance in colon carcinoma. Cytotechnology, 46 1: 9-18. doi:10.1007/s10616-005-1476-5


Author Timmins, N. E.
Maguire, T. L.
Grimmond, S. M.
Nielsen, L. K.
Title Identification of three gene candidates for multicellular resistance in colon carcinoma
Journal name Cytotechnology   Check publisher's open access policy
ISSN 0920-9069
1573-0778
Publication date 2004-09-01
Year available 2005
Sub-type Article (original research)
DOI 10.1007/s10616-005-1476-5
Volume 46
Issue 1
Start page 9
End page 18
Total pages 10
Editor D W Barnes (Managing Editor)
Otto-Wilhelm Merten (Managing Editor)
Sanetaka Shirahata (Managing Editor)
Place of publication Dordrecht
Publisher Springer-Verlag Dordrecht
Collection year 2005
Language eng
Subject C1
270303 Virology
770804 Control of pests and exotic species
Abstract Solid tumours display elevated resistance to chemo- and radiotherapies compared to individual tumour derived cells. This so-called multicellular resistance (MCR) phenomenon can only be partly explained by reduced diffusion and altered cell cycle status; even fast growing cells on the surface of solid tumours display MCR. Multicellular spheroids (MCS) recapture this phenomenon ex vivo and here we compare gene expression in exponentially growing MCS with gene expression in monolayer culture. Using an 18,664 gene microarray, we identified 42 differentially expressed genes and three of these genes can be linked to potential mechanisms of MCR. A group of interferon response genes were also up-regulated in MCS, as were a number of genes that that are indicative of greater differentiation in three-dimensional cultures.
Keyword Biotechnology & Applied Microbiology
Cell Biology
3d Culture
Differential Gene Expression
Mcr
Mcs
Multicellular Resistance
Multicellular Spheroid
Tumour
Nf-kappa-b
Differential Expression
Confers Resistance
Growth-kinetics
Tumor Spheroids
Cancer-cells
Transcription
Overexpression
Environment
Monolayer
Q-Index Code C1
Additional Notes Journal published in 2005 and backdated

 
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Created: Wed, 15 Aug 2007, 17:44:59 EST