A novel carbonic anhydrase from the giant clam Tridacna gigas contains two carbonic anhydrase domains

Leggat, W, Dixon, R, Saleh, S and Yellowlees, D (2005) A novel carbonic anhydrase from the giant clam Tridacna gigas contains two carbonic anhydrase domains. Febs Journal, 272 13: 3297-3305.


Author Leggat, W
Dixon, R
Saleh, S
Yellowlees, D
Title A novel carbonic anhydrase from the giant clam Tridacna gigas contains two carbonic anhydrase domains
Journal name Febs Journal   Check publisher's open access policy
ISSN 1742-464X
Publication date 2005
Sub-type Article (original research)
DOI 10.1111/j.1742-4658.2005.04742.x
Volume 272
Issue 13
Start page 3297
End page 3305
Total pages 9
Editor Richard Perham
Place of publication Oxford, UK
Publisher Blackwell
Collection year 2005
Language eng
Subject C1
270601 Animal Physiology - Biophysics
770300 Marine Environment
Abstract This report describes the presence of a unique dual domain carbonic anhydrase (CA) in the giant clam, Tridacna gigas. CA plays an important role in the movement of inorganic carbon (C-i) from the surrounding seawater to the symbiotic algae that are found within the clam's tissue. One of these isoforms is a glycoprotein which is significantly larger (70 kDa) than any previously reported from animals (generally between 28 and 52 kDa). This alpha-family CA contains two complete carbonic anhydrase domains within the one protein, accounting for its large size; dual domain CAs have previously only been reported from two algal species. The protein contains a leader sequence, an N-terminal CA domain and a C-terminal CA domain. The two CA domains have relatively little identity at the amino acid level (29%). The genomic sequence spans in excess of 17 kb and contains at least 12 introns and 13 exons. A number of these introns are in positions that are only found in the membrane attached/secreted CAs. This fact, along with phylogenetic analysis, suggests that this protein represents the second example of a membrane attached invertebrate CA and it contains a dual domain structure unique amongst all animal CAs characterized to date.
Keyword Biochemistry & Molecular Biology
Carbonic Anhydrase
Clam
Symbiosis
2-domain Arginine Kinase
Amino-acid-sequence
Gene Duplication
Evolution
Prediction
Midgut
Fusion
Cdna
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2006 Higher Education Research Data Collection
Centre for Marine Studies Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 4 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 4 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Access Statistics: 39 Abstract Views  -  Detailed Statistics
Created: Wed, 15 Aug 2007, 07:40:35 EST