Cholesterol and fatty acids regulate dynamic caveolin trafficking through the golgi complex and between the cell surface and lipid bodies

Pol, Albert, Martin, Sally, Fernandez, Manuel A., Ingelmo-Torres, M., Ferguson, C., Enrich, C. and Parton, R. G. (2005) Cholesterol and fatty acids regulate dynamic caveolin trafficking through the golgi complex and between the cell surface and lipid bodies. Molecular Biology of The Cell, 16 4: 2091-2105. doi:10.1091/mbc.E04-08-0737

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Author Pol, Albert
Martin, Sally
Fernandez, Manuel A.
Ingelmo-Torres, M.
Ferguson, C.
Enrich, C.
Parton, R. G.
Title Cholesterol and fatty acids regulate dynamic caveolin trafficking through the golgi complex and between the cell surface and lipid bodies
Journal name Molecular Biology of The Cell   Check publisher's open access policy
ISSN 1059-1524
1939-4586
Publication date 2005
Sub-type Article (original research)
DOI 10.1091/mbc.E04-08-0737
Open Access Status File (Publisher version)
Volume 16
Issue 4
Start page 2091
End page 2105
Total pages 15
Place of publication Bethesda
Publisher American Society for Cell Biology
Collection year 2005
Language eng
Subject C1
270104 Membrane Biology
780106 Political science and public policy
Abstract Caveolins are a crucial component of plasma membrane (PM) caveolae but have also been localized to intracellular compartments, including the Golgi complex and lipid bodies. Mutant caveolins associated with human disease show aberrant trafficking to the PM and Golgi accumulation. We now show that the Golgi pool of mainly newly synthesized protein is detergent-soluble and predominantly in a monomeric state, in contrast to the surface pool. Caveolin at the PM is not recognized by specific caveolin antibodies unless PM cholesterol is depleted. Exit from the Golgi complex of wild-type caveolin-1 or -3, but not vesicular stomatitis virus-G protein, is modulated by changing cellular cholesterol levels. In contrast, a muscular dystrophy-associated mutant of caveolin-3, Cav3P104L, showed increased accumulation in the Golgi complex upon cholesterol treatment. In addition, we demonstrate that in response to fatty acid treatment caveolin can follow a previously undescribed pathway from the PM to lipid bodies and can move from lipid bodies to the PM in response to removal of fatty acids. The results suggest that cholesterol is a rate-limiting component for caveolin trafficking. Changes in caveolin flux through the exocytic pathway can therefore be an indicator of cellular cholesterol and fatty acid levels.
Keyword Cell Biology
Muscular-dystrophy Lgmd-1c
Dominant-negative Mutant
Molecular Characterization
Membrane Microdomains
Gene-transcription
3t3-l1 Adipocytes
Epithelial-cells
Binding Protein
Mdck Cells
In-vivo
Q-Index Code C1

 
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Created: Wed, 15 Aug 2007, 07:14:16 EST