The Runx1 transcription factor controls CSF-1-dependent and -independent growth and survival of macrophages

Himes, S. R., Cronau, S., Mulford, C. and Hume, D. A. (2005) The Runx1 transcription factor controls CSF-1-dependent and -independent growth and survival of macrophages. Oncogene, 24 34: 5278-5286. doi:10.1038/sj.onc.1208657

Author Himes, S. R.
Cronau, S.
Mulford, C.
Hume, D. A.
Title The Runx1 transcription factor controls CSF-1-dependent and -independent growth and survival of macrophages
Journal name Oncogene   Check publisher's open access policy
ISSN 0950-9232
Publication date 2005
Sub-type Article (original research)
DOI 10.1038/sj.onc.1208657
Volume 24
Issue 34
Start page 5278
End page 5286
Total pages 9
Place of publication London, UK
Publisher Nature Publishing Group
Collection year 2005
Language eng
Subject C1
320202 Cellular Immunology
730102 Immune system and allergy
Abstract Gene translocations that repress the function of the Runx1 transcription factor play a critical role in the development of myeloid leukemia. In this report, we demonstrate that Runx1 precisely regulates c-fms (CSF-1 receptor) gene expression. Runx1 controlled expression by binding to multiple sites within the mouse c-fms gene, allowing interaction between promoter and downstream enhancer elements. The runx1 and c-fms genes showed an identical pattern of expression in mature macrophages. Runx1 expression was repressed in CSF-1 stimulated, proliferating bone marrow-derived macrophages (BMM) and significantly increased in quiescent, CSF-1 starved cells. The RAW264.7 and Mono-Mac-6, macrophage-like cell lines expressed low levels of Runx1 and both showed growth arrest and cell death with ectopic expression of Runx1. The EM-3 cell line, which represents an early myeloid progenitor cell line, showed growth arrest with Runx1 expression in the absence of any detectable changes in cell differentiation. These findings suggest that Runx1 regulates growth and survival of myeloid cells and provide a novel insight into the role of Runx family gene translocations in leukemogenesis.
Keyword Biochemistry & Molecular Biology
Cell Biology
Genetics & Heredity
Acute Myelomonocytic Leukemia
Aml1/eto Fusion Transcript
Acute Myelogenous Leukemia
Fetal Liver Hematopoiesis
Acute Myeloid-leukemia
Myosin Heavy-chain
Factor Beta
Definitive Hematopoiesis
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2006 Higher Education Research Data Collection
Institute for Molecular Bioscience - Publications
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Citation counts: TR Web of Science Citation Count  Cited 22 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 21 times in Scopus Article | Citations
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Created: Wed, 15 Aug 2007, 07:09:03 EST