Field evaluation of anthelmintic drug sensitivity using in vitro egg hatch and larval motility assays with Necator americanus recovered from human clinical isolates

Kotze, A. C., Coleman, G. T., Mai, A. and McCarthy, J. S. (2005) Field evaluation of anthelmintic drug sensitivity using in vitro egg hatch and larval motility assays with Necator americanus recovered from human clinical isolates. International Journal For Parasitology, 35 4: 445-453. doi:10.1016/j.ijpara.2004.12.007


Author Kotze, A. C.
Coleman, G. T.
Mai, A.
McCarthy, J. S.
Title Field evaluation of anthelmintic drug sensitivity using in vitro egg hatch and larval motility assays with Necator americanus recovered from human clinical isolates
Journal name International Journal For Parasitology   Check publisher's open access policy
ISSN 0020-7519
Publication date 2005-04-01
Sub-type Article (original research)
DOI 10.1016/j.ijpara.2004.12.007
Volume 35
Issue 4
Start page 445
End page 453
Total pages 9
Editor N. Sangster
Place of publication UK
Publisher Elsevier
Collection year 2005
Language eng
Subject C1
300508 Parasitology
780105 Biological sciences
06 Biological Sciences
070205 Animal Protection (Pests and Pathogens)
Abstract A field-applicable assay for testing anthelmintic sensitivity is required to monitor for anthelmintic resistance. We undertook a study to evaluate the ability of three in vitro assay systems to define drug sensitivity of clinical isolates of the human hookworm parasite Necator americanus recovered from children resident in a village in Madang Province, Papua New Guinea. The assays entailed observation of drug effects on egg hatch (EHA), larval development (LDA), and motility of infective stage larvae (LMA). The egg hatch assay proved the best method for assessing the response to benzimidazole anthelmintics, while the larval motility assay was suitable for assessing the response to ivermectin. The performance of the larval development assay was unsatisfactory on account of interference caused by contaminating bacteria. A simple protocol was developed whereby stool samples were subdivided and used for immediate egg recovery, as well as for faecal culture, in order to provide eggs and infective larvae, respectively, for use in the egg hatch assay and larval motility assay systems. While the assays proved effective in quantifying drug sensitivity in larvae of the drug-susceptible hookworms examined in this study, their ability to indicate drug resistance in larval or adult hookworms remains to be determined. (c) 2005 Australian Society for Parasitology Inc. Published by Elsevier Ltd. All rights reserved.
Keyword Anthelmintic Drug Sensitivity
Egg Hatch
Larval Motility
Human Isolates
Parasitology
Necator Americanus
Drug Sensitivity
Ivermectin
Benzimidazole
Resistance
Haemonchus-contortus
Human Hookworm
Nematodes
Efficacy
Program
Q-Index Code C1

 
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Created: Wed, 15 Aug 2007, 17:07:51 EST