Chloroplast and total genomic diversity in the endemic Costa Rican tree Lonchocarpus costaricensis (J.D. Smith) Pittier (Papilionaceae).

Navarro, C, Cavers, S, Colpaert, N, Hernandez, G, Breyne, P and Lowe, AJ (2005) Chloroplast and total genomic diversity in the endemic Costa Rican tree Lonchocarpus costaricensis (J.D. Smith) Pittier (Papilionaceae).. Silvae Genetica, 54 6: 293-300.

Author Navarro, C
Cavers, S
Colpaert, N
Hernandez, G
Breyne, P
Lowe, AJ
Title Chloroplast and total genomic diversity in the endemic Costa Rican tree Lonchocarpus costaricensis (J.D. Smith) Pittier (Papilionaceae).
Journal name Silvae Genetica   Check publisher's open access policy
ISSN 0037-5349
Publication date 2005
Sub-type Article (original research)
Volume 54
Issue 6
Start page 293
End page 300
Total pages 8
Editor H J Muhs
Place of publication Germany
Publisher J D Sauerlaender's Verlag
Collection year 2005
Language eng
Subject C1
270203 Population and Ecological Genetics
771006 Remnant vegetation and protected conservation areas
Abstract In Mesoamerica, tropical dry forest is a highly threatened habitat, and species endemic to this environment are under extreme pressure. The tree species, Lonchocarpus costaricensis is endemic to the dry northwest of Costa Rica and southwest Nicaragua. It is a locally important species but, as land has been cleared for agriculture, populations have experienced considerable reduction and fragmentation. To assess current levels and distribution of genetic diversity in the species, a combination of chloroplast-specific (cpDNA) and whole genome DNA markers (amplified fragment length polymorphism, AFLP) were used to fingerprint 121 individual trees in 6 populations. Two cpDNA haplotypes were identified, distributed among populations such that populations at the extremes of the distribution showed lowest diversity. A large number (487) of AFLP markers were obtained and indicated that diversity levels were highest in the two coastal populations (Cobano, Matapalo, H = 0.23, 0.28 respectively). Population differentiation was low overall, F-ST = 0.12, although Matapalo was strongly differentiated from all other populations (F-ST = 0.16-0.22), apart from Cobano (F., = 0.11). Spatial genetic structure was present in both datasets at different scales: cpDNA was structured at a range-wide distribution scale, whilst AFLP data revealed genetic neighbourhoods on a population scale. In general, the habitat degradation of recent times appears not to have yet impacted diversity levels in mature populations. However, although no data on seed or saplings were collected, it seems likely that reproductive mechanisms in the species will have been affected by land clearance. It is recommended that efforts should be made to conserve the extant genetic resource base and further research undertaken to investigate diversity levels in the progeny generation.
Keyword Forestry
Genetics & Heredity
Chloroplast Dna
Genetic Differentiation
Genetic Diversity
Lonchocarpus Costaricensis
Spatial Genetic Structuring
Noncoding Regions
Universal Primers
Dna Markers
Dry Forest
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2006 Higher Education Research Data Collection
School of Biological Sciences Publications
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Created: Wed, 15 Aug 2007, 07:07:44 EST