Sampling tissue for DNA analysis of trees: Trunk cambium as an alternative to canopy leaves

Colpaert, N., Cavers, S., Bandou, E., Caron, H., Gheysen, G. and Lowe, A. J. (2005) Sampling tissue for DNA analysis of trees: Trunk cambium as an alternative to canopy leaves. Silvae Genetica, 54 6: 265-269.


Author Colpaert, N.
Cavers, S.
Bandou, E.
Caron, H.
Gheysen, G.
Lowe, A. J.
Title Sampling tissue for DNA analysis of trees: Trunk cambium as an alternative to canopy leaves
Journal name Silvae Genetica   Check publisher's open access policy
ISSN 0037-5349
Publication date 2005
Sub-type Article (original research)
Volume 54
Issue 6
Start page 265
End page 269
Total pages 5
Place of publication Germany
Publisher J. D. Sauerlaender's Verlag
Collection year 2005
Language eng
Subject C1
270203 Population and Ecological Genetics
771006 Remnant vegetation and protected conservation areas
0604 Genetics
0705 Forestry Sciences
Abstract The number of studies of tropical tree species that use molecular tools is increasing, most of which collect leaf tissue for genomic DNA extraction. In tropical trees the canopy is not only frequently inaccessible, but also, once reached, the leaf tissue is often heavily defended against herbivory by high concentrations of anti-predation compounds, which may inhibit downstream applications, particularly PCR. Cambium tissue, accessed directly from the tree trunk at ground level, offers a readily accessible resource that is less hampered by the presence of defensive chemicals than leaf tissue. Here we describe a simple method for obtaining tissue from the cambial zone for DNA extraction and test the applicability of the method in a range of tropical tree species. The method was used successfully to extract DNA from 11 species in nine families. A subset of the DNA extracts was tested in more detail and proved to be highly suitable for AFLP analysis.
Keyword Forestry
Genetics & Heredity
Dna Extraction
Trees
Tropical
Cambium
Aflp
Sample Preservation
Costa-rican Populations
Herbivory
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2006 Higher Education Research Data Collection
School of Biological Sciences Publications
 
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Created: Wed, 15 Aug 2007, 07:07:14 EST