Neural Stem Cells and Neurospheres - Re-evaluating the Relationship

Reynolds, Brent A. and Rietze, Rodney L. (2005) Neural Stem Cells and Neurospheres - Re-evaluating the Relationship. Nature Methods, 2 5: 333-336. doi:10.1038/nmeth758

Author Reynolds, Brent A.
Rietze, Rodney L.
Title Neural Stem Cells and Neurospheres - Re-evaluating the Relationship
Journal name Nature Methods   Check publisher's open access policy
ISSN 1548-7091
Publication date 2005-05
Sub-type Article (original research)
DOI 10.1038/nmeth758
Volume 2
Issue 5
Start page 333
End page 336
Total pages 4
Editor Veronique Kiermer
Place of publication London
Publisher Nature Publishing Group
Collection year 2005
Language eng
Subject C1
320702 Central Nervous System
730104 Nervous system and disorders
Abstract For most of the past century, the prospect of replacing lost or damaged cells in the central nervous system (CNS) was hampered by the opinion that the adult mammalian CNS was incapable of generating new nerve cells. This belief, Like most dogmas, was essentially founded on a lack of experimental evidence to the contrary. The overturning of this 'no new neuron' hypothesis began midway through the twentieth century with a series of reports documenting neurogenesis in the postnatal and adult brain(1), continued with the isolation and in vitro culture of neurogenic cells from the adult mammalian brain(2,3), and culminated in the discovery of a population of muttipotent, selfrenewing cells in the adult CNS (that is, bona fide neural stem cells)(3-5). Although a variety of techniques were initially used, the neurosphere assay (NSA)(3,6) rapidly emerged as the assay of choice and has since become a valuable toot for isolating, and understanding the biology of, embryonic and adult CNS stem cells. Like all technologies, it is not without its limitations. In this article we will hightight several shortcomings of the assay related to its application and interpretation that we believe have led to a significant body of research whose conclusions may well be misleading.
Keyword Biochemical Research Methods
Mammalian Forebrain
neural stem cell
Q-Index Code C1

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Created: Wed, 15 Aug 2007, 07:04:08 EST