Protein adduct species in muscle and liver of rats following acute ethanol administration

Patel, Vinood B., Worrall, Simon, Emery, Peter W. and Preedy, Victor R. (2005) Protein adduct species in muscle and liver of rats following acute ethanol administration. Alcohol and Alcoholism, 40 6: 485-493. doi:10.1093/alcalc/agh196

Author Patel, Vinood B.
Worrall, Simon
Emery, Peter W.
Preedy, Victor R.
Title Protein adduct species in muscle and liver of rats following acute ethanol administration
Journal name Alcohol and Alcoholism   Check publisher's open access policy
ISSN 0735-0414
Publication date 2005
Sub-type Article (original research)
DOI 10.1093/alcalc/agh196
Open Access Status DOI
Volume 40
Issue 6
Start page 485
End page 493
Total pages 9
Place of publication Oxford
Publisher Oxford University Press
Collection year 2005
Language eng
Subject C1
270199 Biochemistry and Cell Biology not elsewhere classified
730118 Organs, diseases and abnormal conditions not elsewhere classified
Abstract Aims: Previous immunohistochemical studies have shown that the post-translational formation of aldehyde-protein adducts may be an important process in the aetiology of alcohol-induced muscle disease. However, other studies have shown that in a variety of tissues, alcohol induces the formation of various other adduct species, including hybrid acetaldehyde-malondialdehyde-protein adducts and adducts with free radicals themselves, e.g. hydroxyethyl radical (HER)-protein adducts. Furthermore, acetaldehyde-protein adducts may be formed in reducing or non-reducing environments resulting in distinct molecular entities, each with unique features of stability and immunogenicity. Some in vitro studies have also suggested that unreduced adducts may be converted to reduced adducts in situ. Our objective was to test the hypothesis that in muscle a variety of different adduct species are formed after acute alcohol exposure and that unreduced adducts predominate. Methods: Rabbit polyclonal antibodies were raised against unreduced and reduced aldehydes and the HER-protein adducts. These were used to assay different adduct species in soleus (type I fibre-predominant) and plantaris (type II fibre-predominant) muscles and liver in four groups of rats administered acutely with either [A] saline (control); [B] cyanamide (an aldehyde dehydrogenase inhibitor); [C] ethanol; [D] cyanamide+ethanol. Results: Amounts of unreduced acetaldehyde and malondialdehyde adducts were increased in both muscles of alcohol-dosed rats. However there was no increase in the amounts of reduced acetaldehyde adducts, as detected by both the rabbit polyclonal antibody and the RT1.1 mouse monoclonal antibody. Furthermore, there was no detectable increase in malondialdehyde-acetaldehyde and HER-protein adducts. Similar results were obtained in the liver. Conclusions: Adducts formed in skeletal muscle and liver of rats exposed acutely to ethanol are mainly unreduced acetaldehyde and malondialdehyde species.
Keyword Substance Abuse
Malondialdehyde-acetaldehyde Adducts
Induced Lipid-peroxidation
Chronic-alcoholic Myopathy
Modified Epitopes
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2006 Higher Education Research Data Collection
School of Chemistry and Molecular Biosciences
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Citation counts: TR Web of Science Citation Count  Cited 12 times in Thomson Reuters Web of Science Article | Citations
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Created: Wed, 15 Aug 2007, 07:00:57 EST