Preparation of immuno-stimulating complexes (ISCOMs) by ether injection

Pham, H. L., Shaw, P. N. and Davies, N. M. (2006) Preparation of immuno-stimulating complexes (ISCOMs) by ether injection. International Journal of Pharmaceutics, 310 1-2: 196-202. doi:10.1016/j.ijpharm.2005.11.011


Author Pham, H. L.
Shaw, P. N.
Davies, N. M.
Title Preparation of immuno-stimulating complexes (ISCOMs) by ether injection
Journal name International Journal of Pharmaceutics   Check publisher's open access policy
ISSN 0378-5173
Publication date 2006
Sub-type Article (original research)
DOI 10.1016/j.ijpharm.2005.11.011
Volume 310
Issue 1-2
Start page 196
End page 202
Total pages 7
Editor A. T. Florence
J. H. Rytting
T. Nagai
Place of publication Netherlands
Publisher Elsevier
Collection year 2006
Language eng
Subject 320501 Pharmaceutical Sciences and Pharmacy
730101 Infectious diseases
Abstract preparation of liposomes, as a new, continuous and potentially scaleable method for the preparation of ISCOMs. Phosphatidylcholine (PC) and cholesterol (Chol) were dissolved in ether, which was injected into an aqueous solution, maintained at 55 degrees C, containing Quil A. The influences of the following variables on ISCOM formation were investigated: ratio of PC:Quil A:Chol used, pumping rate, total lipid mass and concentration of buffer salts and Quil A in the aqueous phase. All samples were characterized by negative stain transmission electron microscopy, photon correlation spectroscopy and sucrose ultracentrifugation gradient. It was demonstrated that ISCOMs could be produced by this method but the homogeneity of the preparation was influenced by the conditions used. Homogeneous ISCOM preparations were consistently produced only when the weight ratio of PC:Quil A:Chol was 5:3:2 with a total lipid mass of 20 mg, the Quil A dissolved in a 0.01 M phosphate buffer at a concentration of 6 mg in 4 ml, and the ether solution injected into the warmed buffer solution at a rate of 0.2 ml/min. Changing any of these variables resulted in more heterogeneous preparations in which ISCOMs typically co-existed with other colloidal structures such as worm-like and helical micelles, liposomes, lamellae and lipidic particles. (C) 2005 Elsevier B.V. All rights reserved.
Keyword Pharmacology & Pharmacy
Iscom
Immuno-stimulating Complex
Quil A
Adjuvant
Ether Injection
Lipid-film Hydration
Aqueous Mixtures
Quil-a
Cholesterol
Liposomes
Responses
Dialysis
Diagrams
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
2007 Higher Education Research Data Collection
School of Pharmacy Publications
 
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Created: Wed, 15 Aug 2007, 06:59:06 EST