A rapid HPLC method with fluorometric detection for determination of plasma itraconazole and hydroxyitraconazole concentrations in cystic fibrosis children with allergic bronchopulmonary aspergillosis

Redmann, S. and Charles, B. G. (2006) A rapid HPLC method with fluorometric detection for determination of plasma itraconazole and hydroxyitraconazole concentrations in cystic fibrosis children with allergic bronchopulmonary aspergillosis. Biomedical Chromatography, 20 4: 343-348.


Author Redmann, S.
Charles, B. G.
Title A rapid HPLC method with fluorometric detection for determination of plasma itraconazole and hydroxyitraconazole concentrations in cystic fibrosis children with allergic bronchopulmonary aspergillosis
Journal name Biomedical Chromatography   Check publisher's open access policy
ISSN 0269-3879
1099-0801
Publication date 2006-04
Year available 2005
Sub-type Article (original research)
DOI 10.1002/bmc.569
Volume 20
Issue 4
Start page 343
End page 348
Total pages 6
Editor C. K. Lim
Place of publication Bognor Regis, West Sussex, U.K.
Publisher John Wiley & Sons
Collection year 2006
Language eng
Subject C1
320503 Clinical Pharmacology and Therapeutics
730101 Infectious diseases
Formatted abstract The development and validation of a simple, rapid and selective high-performance liquid chromatography (HPLC) method is described for the quantitation of itraconazole and hydroxy-itraconazole in 100 µL of plasma from a paediatric population. The mobile phase of methanol (75% v/v) and water (25% v/v) was pumped at 1 mL/min through a C18Symmetry (3.9 mm i.d. × 150 mm) cartridge. Using a protein-precipitation method, 100 µL internal standard (IS) solution (R051012, 555 µg/L in acetonitrile) were added to 100 µL of plasma followed by 10 µL zinc sulphate solution (20% w/v). Itraconazole, hydroxy-itraconazole and IS eluted at 4.7, 8.3 and 12.5 min, respectively and were detected ßuorometrically at 250 nm (excitation) and 380 nm (emission). Recoveries were 87.1–96.7%. Calibrations in drug-free plasma were linear (r2 > 0.99) from 50 to 2000 µg/L, using 1/c2 (c = concentration) weighting. Intraday and interday imprecision (CV%) was 4.8–17.3 and 6.3–16.6% for itraconazole, and 4.6–17.9 and 7.02–18.4% for hydroxy-itraconazole. Inaccuracy was −7.1 to −14.7% for itraconazole and −0.1 to −9.7% for hydroxy-itraconazole. The clinical application of this method was demonstrated by measurement of itraconazole and hydroxy-itraconazole in plasma samples drawn from paediatric cystic fibrosis patients, who were prescribed itraconazole for treatment of allergic bronchopulmonary aspergillosis.
Keyword Biochemical Research Methods
Biochemistry & Molecular Biology
Chemistry, Analytical
Pharmacology & Pharmacy
Itraconazole
Hydroxy-itraconazole
High-performance Liquid Chromatography (hplc)
Cystic Fibrosis
Performance Liquid-chromatography
Solid-phase Extraction
Transplant Recipients
Hydroxy-metabolite
Mass-spectrometry
P-glycoprotein
Assay
Cyclosporine
Digoxin
Serum
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ
Additional Notes Article first published online: 14 SEP 2005

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
2007 Higher Education Research Data Collection
School of Pharmacy Publications
 
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