A rapid and sensitive microscale HPLC method for the determination of indomethacin in plasma of premature neonates with patent ductus arteriousus

Al Za'abi, MA, Dehghanzadeh, GH, Norris, RLG and Charles, BG (2006) A rapid and sensitive microscale HPLC method for the determination of indomethacin in plasma of premature neonates with patent ductus arteriousus. Journal of Chromatography B-analytical Technologies In The Biomedical And Life Sciences, 830 2: 364-367. doi:10.1016/j.jchromb.2005.11.025


Author Al Za'abi, MA
Dehghanzadeh, GH
Norris, RLG
Charles, BG
Title A rapid and sensitive microscale HPLC method for the determination of indomethacin in plasma of premature neonates with patent ductus arteriousus
Journal name Journal of Chromatography B-analytical Technologies In The Biomedical And Life Sciences   Check publisher's open access policy
ISSN 1570-0232
Publication date 2006
Sub-type Article (original research)
DOI 10.1016/j.jchromb.2005.11.025
Volume 830
Issue 2
Start page 364
End page 367
Total pages 4
Editor G. Hopfgartner
H.T. Karnes
R. Bischoff
Place of publication Netherlands
Publisher Elsevier BV
Collection year 2006
Language eng
Subject C1
320503 Clinical Pharmacology and Therapeutics
730106 Cardiovascular system and diseases
Abstract Indomethacin (IND) is the drug of choice for the closure of a patent ductus arteriosus (PDA) in neonates. This paper describes a simple, sensitive, accurate and precise microscale HPLC method suitable for the analysis of IND in plasma of premature neonates. Samples were prepared by plasma protein precipitation with acetonitrile containing the methyl ester of IND as the internal standard (IS). Chromatography was performed on a Hypersil C-18 column. The mobile phase of methanol, water and orthophosphoric acid (70:29.5:0.5, v/v, respectively), was delivered at 1.5 mL/min and monitored at 270 nm. IND and the IS were eluted at 2.9 and 4.3 min, respectively. Calibrations were linear (r > 0.999) from 25 to 2500 mu g/L. The inter- and intra-day assay imprecision was less than 4.3% at 400-2000 mu g/L, and less than 22.1% at 35 mu g/L. Inaccuracy ranged from -6.0% to +1.0% from 35 to 2000 mu g/L. The absolute recovery of IND over this range was 93.0-113.3%. The IS was stable for at least 36 h when added to plasma at ambient temperature. This method is suitable for pharmacokinetic studies of IND and has potential for monitoring therapy in infants with PDA when a target therapeutic range for IND has been validated. (c) 2005 Elsevier B.V. All rights reserved.
Keyword Biochemical Research Methods
Chemistry, Analytical
Indomethacin
Patent Ductus Arteriosus
Premature Neonates
Hplc
Performance Liquid-chromatography
Preterm Infants
Serum
Disposition
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
2007 Higher Education Research Data Collection
School of Pharmacy Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 13 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 17 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Access Statistics: 311 Abstract Views  -  Detailed Statistics
Created: Wed, 15 Aug 2007, 06:55:23 EST