Testicular development of Zebu bulls after chronic treatment with a gonadotropin-releasing hormone agonist

Jimenez-Severiano, H, Mussard, ML, Fitzpatrick, LA, D'Occhio, MJ, Ford, JJ, Lunstra, DD and Kinder, JE (2005) Testicular development of Zebu bulls after chronic treatment with a gonadotropin-releasing hormone agonist. Journal of Animal Science, 83 9: 2111-2122.

Author Jimenez-Severiano, H
Mussard, ML
Fitzpatrick, LA
D'Occhio, MJ
Ford, JJ
Lunstra, DD
Kinder, JE
Title Testicular development of Zebu bulls after chronic treatment with a gonadotropin-releasing hormone agonist
Journal name Journal of Animal Science   Check publisher's open access policy
ISSN 0021-8812
Publication date 2005
Sub-type Article (original research)
Open Access Status
Volume 83
Issue 9
Start page 2111
End page 2122
Total pages 12
Editor John Edwards
Jean Rice
Place of publication United States
Publisher American Society of Animal Science
Collection year 2005
Language eng
Subject C1
300402 Animal Reproduction
630103 Beef cattle
Abstract The objective was to compare testis characteristics of Zebu bulls treated with the GnRH agonist, deslorelin, at different times and for different durations during their development. An additional objective was to determine the usefulness of a stain for the transcription factor GATA-binding protein 4 (GATA-4) as a specific marker for Sertoli cell nuclei in cattle. Bulls (54) were allocated to nine groups (n = 6) and received s.c. deslorelin implants as follows: G1 = from birth to 3 mo of age; G2 = from 3 to 6 mo; G3 = from 6 to 9 mo; G4 = from 9 to 12 mo; G5 = from birth to 15 mo; G6 = from 3 to 15 mo; G7 = from 6 to 15 mo; G8 = from 12 to 15 mo; and G9 (control) = no implant. Bulls were castrated at 19 mo of age. Paraffin sections (10 mu m) were subjected to quantitative morphometry and GATA-4 immunohistochemistry. At castration, all bulls in the control group (6/6) had attained puberty (scrotal circumference ! 28 cm), whereas a smaller proportion (P < 0.05) had reached puberty in G2 (2/5) and G6 (1/ 6). Bulls in G2 and G6 also had a lesser (P < 0.05) testis weight compared with the control group. Total volume of seminiferous epithelium and total daily sperm production in G2 and G6 were only half that observed in the control group. Spermatids were observed in less than 50% of seminiferous tubules in G2, G6, and G7 compared with 82% in the control group (P < 0.05). Staining for GATA-4 was specific for and abundant in the Sertoli cell nucleus in both pre- and postpubertal bulls, and no other cell nucleus inside the seminiferous tubule was positive for GATA-4. Total number of Sertoli cells was not affected by treatment (P = 0.45), but nuclear volume was smaller in G2 and G6 (P < 0.05) compared with the control group. In conclusion, treatment of Zebu bulls with deslorelin had no apparent beneficial effect on testis development and delayed puberty when treatment was initiated at 3 mo of age. Staining for GATA-4 was a useful method for identifying and quantifying Sertoli cell nuclei in both pre- and postpubertal bulls.
Keyword Agriculture, Dairy & Animal Science
Gata-binding Protein 4
Gonadotropin-releasing Hormone
Male Sexual Function
Sertoli Cells
Testis Development
Zebu Cattle
Beef Bulls
Testosterone Secretion
Sperm Production
Irradiated Rats
Holstein Bulls
Young Bulls
Q-Index Code C1

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Created: Wed, 15 Aug 2007, 06:50:42 EST