Identification and analysis of venom gland-specific genes from the coastal taipan (Oxyuranus scutellatus) and related species

St Pierre, L., Woods, R., Earl, S., Masci, P. P. and Lavin, M. F. (2005) Identification and analysis of venom gland-specific genes from the coastal taipan (Oxyuranus scutellatus) and related species. Cellular And Molecular Life Sciences, 62 22: 2679-2693. doi:10.1007/s00018-005-5384-9


Author St Pierre, L.
Woods, R.
Earl, S.
Masci, P. P.
Lavin, M. F.
Title Identification and analysis of venom gland-specific genes from the coastal taipan (Oxyuranus scutellatus) and related species
Journal name Cellular And Molecular Life Sciences   Check publisher's open access policy
ISSN 1420-682X
Publication date 2005-01-01
Sub-type Article (original research)
DOI 10.1007/s00018-005-5384-9
Volume 62
Issue 22
Start page 2679
End page 2693
Total pages 15
Editor P. Jolles
Place of publication Switzerland
Publisher Birkhaeuser Verlag AG
Collection year 2005
Language eng
Subject C1
321206 Preventive Medicine
730220 Injury control
Abstract Australian terrestrial elapid snakes contain amongst the most potently toxic venoms known. However, despite the well-documented clinical effects of snake bite, little research has focussed on individual venom components at the molecular level. To further characterise the components of Australian elapid venoms, a complementary (cDNA) microarray was produced from the venom gland of the coastal taipan (Oxyuranus scutellatus) and subsequently screened for venom gland-specific transcripts. A number of putative toxin genes were identified, including neurotoxins, phospholipases, a pseudechetoxin-like gene, a venom natriuretic peptide and a nerve growth factor together with other genes involved in cellular maintenance. Venom gland-specific components also included a calglandulin-like protein implicated in the secretion of toxins from the gland into the venom. These toxin transcripts were subsequently identified in seven other related snake species, producing a detailed comparative analysis at the cDNA and protein levels. This study represents the most detailed description to date of the cloning and characterisation of different genes associated with envenomation from Australian snakes.
Keyword Biochemistry & Molecular Biology
Cell Biology
Gene Cloning
Australian Elapid
Oxyuranus Scutellatus
Pseudechetoxin
Calglandulin
Phospholipase A(2)
L-amino Acid Oxidase
Amino-acid-sequences
Gated Ion Channels
Potent Presynaptic Neurotoxin
Phospholipase A(2) Enzymes
Notechis-scutatus-scutatus
Pre-synaptic Neurotoxin
Australian King Brown
Ef-hand Protein
Snake-venom
S-scutellatus
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
2006 Higher Education Research Data Collection
School of Medicine Publications
 
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Created: Wed, 15 Aug 2007, 16:50:20 EST