Enhanced proliferation, attachment and osteopontin expression by porcine periodontal cells exposed to Emdogain (R)

Rincon, J. C., Xiao, Y., Young, W. G. and Bartold, P. M. (2005) Enhanced proliferation, attachment and osteopontin expression by porcine periodontal cells exposed to Emdogain (R). Archives of Oral Biology, 50 12: 1047-1054. doi:10.1016/j.archoralbio.2005.04.006


Author Rincon, J. C.
Xiao, Y.
Young, W. G.
Bartold, P. M.
Title Enhanced proliferation, attachment and osteopontin expression by porcine periodontal cells exposed to Emdogain (R)
Formatted title
Enhanced proliferation, attachment and osteopontin expression by porcine periodontal cells exposed to Emdogain®
Journal name Archives of Oral Biology   Check publisher's open access policy
ISSN 0003-9969
1879-1506
Publication date 2005-12
Sub-type Article (original research)
DOI 10.1016/j.archoralbio.2005.04.006
Volume 50
Issue 12
Start page 1047
End page 1054
Total pages 8
Editor D. B. Ferguson
E. J. Kollar
Place of publication Oxford, U.K.
Publisher Pergamon Press; ELsevier
Collection year 2005
Language eng
Subject C1
320899 Dentistry not elsewhere classified
730112 Oro-dental and disorders
0608 Zoology
1105 Dentistry
Formatted abstract
Emdogain® (EMD) is an enamel matrix derivative extracted from developing porcine teeth with demonstrated periodontal regenerative potential. EMD has been shown to influence a number of properties of periodontal ligament cells including proliferation, cell attachment and matrix synthesis. To date, the effect of EMD on the epithelial cell rests of Malassez (ERM) is unknown. In this study, periodontal ligament fibroblasts, ERM, alveolar bone cells and gingival fibroblasts were obtained from porcine periodontal ligament, alveolar bone and gingiva. This study investigated, in vitro, the effect of EMD at three concentrations on proliferation, cell attachment and expression of mRNA for two mineralised tissue-related proteins (osteopontin and bone sialoprotein). As for other periodontal cells, the ERM proliferative response was enhanced by EMD. Attachment assays revealed a highly significant increase for ERM and gingival fibroblasts after EMD treatment at all concentrations. This study has also shown that EMD stimulated expression of osteopontin mRNA by ERM and alveolar bone cells. The results from this study provide evidence that EMD enhanced cellular events related with proliferation, attachment and osteopontin mRNA expression by porcine periodontal cells, in a manner consistent with its role in periodontal regenerative therapy.
© 2005 Elsevier Ltd. All rights reserved.
Keyword Dentistry
Epithelial cell rests of Malassez
Osteopontin
Rt-pcr
Emdogain
Attachment
Proliferation
Enamel matrix
In-vitro
Ligament cells
Regeneration
Fibroblasts
Growth
Repair
Proteins
Model
Oral surgery & medicine
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
2006 Higher Education Research Data Collection
School of Dentistry Publications
 
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Created: Wed, 15 Aug 2007, 06:30:43 EST