The combined SPE : ToxY-PAM phytotoxicity assay; application and appraisal of a novel biomonitoring tool for the aquatic environment

Bengtson Nash, S. M., Schreiber, U., Ralph, P. J. and Müller, J. F. (2005) The combined SPE : ToxY-PAM phytotoxicity assay; application and appraisal of a novel biomonitoring tool for the aquatic environment. Biosensors & Bioelectronics, 20 7: 1443-1451. doi:10.1016/j.bios.2004.09.019


Author Bengtson Nash, S. M.
Schreiber, U.
Ralph, P. J.
Müller, J. F.
Title The combined SPE : ToxY-PAM phytotoxicity assay; application and appraisal of a novel biomonitoring tool for the aquatic environment
Journal name Biosensors & Bioelectronics   Check publisher's open access policy
ISSN 0956-5663
1873-4235
Publication date 2005-01-15
Sub-type Article (original research)
DOI 10.1016/j.bios.2004.09.019
Volume 20
Issue 7
Start page 1443
End page 1451
Total pages 9
Editor A. Turner
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Collection year 2005
Language eng
Subject C1
321299 Public Health and Health Services not elsewhere classified
730210 Environmental health
Abstract Mounting concerns regarding the environmental impact of herbicides has meant a growing requirement for accurate, timely information regarding herbicide residue contamination of, in particular, aquatic systems. Conventional methods of detection remain limited in terms of practicality due to high costs of operation and the specialised information that analysis provides. A new phytotoxicity bioassay was trialled for the detection of herbicide residues in filter-purified (Milli-Q) as well as natural waters. The performance of the system, which combines solid-phase extraction (SPE) with the ToxY-PAM dual-channel yield analyser (Heinz Walz GmbH), was tested alongside the traditional method of liquid chromatography-mass spectrometry (LC-MS). The assay methodology was found to be highly sensitive (LOD 0.1 ng L-1 diuron) with good reproducibility. The study showed that the assay protocol is time effective and can be employed for the aquatic screening of herbicide residues in purified as well as natural waters.
Keyword Phytotoxicity
Bioassay
Chlorophyll Fluorescence
Herbicides
Solid-phase Extraction
Environmental Monitoring
Great-barrier-reef
Triazine Herbicides
Quantum Yield
Toxicity
Atrazine
Exposure
Sensitivity
Biosensors
Diuron
Biophysics
Biotechnology & Applied Microbiology
Chemistry, Analytical
Electrochemistry
Nanoscience & Nanotechnology
Q-Index Code C1
Institutional Status UQ

 
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Created: Wed, 15 Aug 2007, 16:27:15 EST