Distribution of the Vitamin D receptor and 1 alpha-hydroxylase in human brain

Eyles, Darryl W., Smith, Steven, Kinobe, Robert, Hewison, Martin and McGrath, John J. (2005) Distribution of the Vitamin D receptor and 1 alpha-hydroxylase in human brain. Journal Of Chemical Neuroanatomy, 29 1: 21-30. doi:10.1016/j.jchemneu.2004.08.006

Author Eyles, Darryl W.
Smith, Steven
Kinobe, Robert
Hewison, Martin
McGrath, John J.
Title Distribution of the Vitamin D receptor and 1 alpha-hydroxylase in human brain
Formatted title
Distribution of the Vitamin D receptor and 1α-hydroxylase in human brain
Journal name Journal Of Chemical Neuroanatomy   Check publisher's open access policy
ISSN 0891-0618
Publication date 2005-01
Year available 2004
Sub-type Article (original research)
DOI 10.1016/j.jchemneu.2004.08.006
Volume 29
Issue 1
Start page 21
End page 30
Total pages 10
Editor Harry W. M. Steinbusch
H. W. M. Steinbusch
R. Quirion
Place of publication Amsterdam
Publisher Elsevier Science
Collection year 2005
Language eng
Subject C1
321204 Mental Health
320702 Central Nervous System
730104 Nervous system and disorders
321021 Psychiatry
Formatted abstract
Despite a growing body of evidence that Vitamin D is involved in mammalian brain functioning, there has been a lack of direct evidence about its role in the human brain. This paper reports, for the first time, the distribution of the 1,25-dihydroxyvitamin D3 receptor (VDR), and 1α-hydroxylase (1α-OHase), the enzyme responsible for the formation of the active vitamin in the human brain. The receptor and the enzyme were found in both neurons and glial cells in a regional and layer-specific pattern. The VDR was restricted to the nucleus whilst 1α-OHase was distributed throughout the cytoplasm. The distribution of the VDR in human brain was strikingly similar to that reported in rodents. Many regions contained equivalent amounts of both the VDR and 1α-OHase, however the macrocellular cells within the nucleus basalis of Meynert (NBM) and the Purkinje cells in the cerebellum expressed 1α-OHase in the absence of VDR. The strongest immunohistochemical staining for both the receptor and enzyme was in the hypothalamus and in the large (presumably dopaminergic) neurons within the substantia nigra. The observed distribution of the VDR is consistent with the proposal that Vitamin D operates in a similar fashion to the known neurosteroids. The widespread distribution of 1α-OHase and the VDR suggests that Vitamin D may have autocrine/paracrine properties in the human brain.
Keyword Biochemistry & Molecular Biology
Vitamin D
1 Alpha-hydroxylase
Human Brain
Brain Development
Nerve Growth-factor
1,25-dihydroxyvitamin D-3
25-hydroxyvitamin D-3-1-alpha-hydroxylase
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ
Additional Notes Available online 1 October 2004.

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Created: Wed, 15 Aug 2007, 06:26:30 EST