New human coronavirus, HCoV-NL63, associated with severe lower respiratory tract disease in Australia

Arden, Katherine E., Nissen, Michael D., Sloots, Theo P. and Mackay, Ian M. (2005) New human coronavirus, HCoV-NL63, associated with severe lower respiratory tract disease in Australia. Journal Of Medical Virology, 75 3: 455-462. doi:10.1002/jmv.20288


Author Arden, Katherine E.
Nissen, Michael D.
Sloots, Theo P.
Mackay, Ian M.
Title New human coronavirus, HCoV-NL63, associated with severe lower respiratory tract disease in Australia
Journal name Journal Of Medical Virology   Check publisher's open access policy
ISSN 0146-6615
ISBN 0146-6615; 1096-9071
Publication date 2005-03
Sub-type Article (original research)
DOI 10.1002/jmv.20288
Volume 75
Issue 3
Start page 455
End page 462
Total pages 8
Editor Arie J Zuckerman
Editor-in-Chief
Place of publication New York, U. S. A.
Publisher Wiley-Liss
Collection year 2005
Language eng
Subject C1
270303 Virology
730101 Infectious diseases
1108 Medical Microbiology
Formatted abstract
A new human coronavirus, HCoV-NL63, was associated recently with bronchiolitis. The current study aimed to examine retrospectively stored specimens for the presence of HCoV-NL63 using nested RT-PCR assays targeting the 1a and 1b genes. The study population was composed of patients with acute respiratory disease warranting presentation to Queensland hospitals. HCoVNL63 was detected in the nasopharyngeal aspirates (NPA) of 16 of 840 specimens representing 766 patients (2%). HCoV-NL63 positive individuals were diagnosed most commonly with lower respiratory tract (LRT) disease (81%). The clinical diagnosis was commonly supported by an abnormal chest X-ray (56%) together with respiratory distress (50%), wheeze (44%), and r (a) over cap les (25%) on first presentation with HCoV-NL63 infection. All patients positive for HCoV-NL63 required admission to hospital. Among 38% of HCoV-NL63 positive specimens a second pathogen was detected. Sequencing of amplicon from gene 1b revealed more than 99% nucleotide homology with the viral type strains while sequencing amplicon from gene 1a permitted the grouping of viral strains. It was shown that HCoV-NL63 is associated with severe LRT disease in an Australian hospital setting during the cooler months of the year. We propose that HCoV-NL63 is a global and seasonal pathogen of both children and adults associated with severe LRT illness.
© 2005 Wiley-Liss, Inc.
Keyword Human coronavirus
Emerging
Respiratory
Pediatric
Disease
Infection
Rhinovirus
Viruses
PCR
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

 
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Created: Wed, 15 Aug 2007, 06:25:56 EST