Expression analysis of a human hepatic cell line in response to palmitate

Swagell, Christopher D., Henly, Debra C. and Morris, C. Phillip (2005) Expression analysis of a human hepatic cell line in response to palmitate. Biochemical and Biophysical Research Communications, 328 2: 432-441. doi:10.1016/j.bbrc.2004.12.188


Author Swagell, Christopher D.
Henly, Debra C.
Morris, C. Phillip
Title Expression analysis of a human hepatic cell line in response to palmitate
Journal name Biochemical and Biophysical Research Communications   Check publisher's open access policy
ISSN 0006-291X
Publication date 2005
Sub-type Article (original research)
DOI 10.1016/j.bbrc.2004.12.188
Volume 328
Issue 2
Start page 432
End page 441
Total pages 10
Place of publication San Diego
Publisher Academic Press Inc Elsevier Science
Collection year 2005
Language eng
Subject C1
270101 Analytical Biochemistry
780105 Biological sciences
Abstract Saturated fat plays a role in common debilitating diseases such as obesity, type 2 diabetes, and coronary heart disease. It is also clear that certain fatty acids act as regulators of metabolism via both direct and indirect signalling of target tissues. As the molecular mechanisms of saturated fatty acid signalling in the liver are poorly defined, hepatic gene expression analysis was undertaken in a human hepatocyte cell line after incubation with palmitate. Profiling of mRNA expression using cDNA microarray analysis revealed that 162 of approximately 18,000 genes tested were differentially expressed after incubation with palmitate for 48 h. Altered transcription profiles were observed in a wide variety of genes, including genes involved in lipid and cholesterol transport, cholesterol catabolism, cell growth and proliferation, cell signalling, P-oxidation, and oxidative stress response. While palinitate signalling has been examined in pancreatic beta-cells, this is the first report showing that palmitate regulates expression of numerous genes via direct molecular signalling mechanisms in liver cells. (C) 2005 Elsevier Inc. All rights reserved.
Keyword Cdna Microarray Analysis
Gene Expression
Saturated Fatty Acid
Palmitate
Hepatocytes
Biochemistry & Molecular Biology
Biophysics
Coronary-heart-disease
High-fat Diet
Cholesterol Efflux
Oxidative Stress
Gene-expression
Transgenic Mice
Growth-factor
Binding
Protein
Liver
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2006 Higher Education Research Data Collection
School of Chemistry and Molecular Biosciences
 
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Created: Wed, 15 Aug 2007, 06:24:27 EST