Transcriptional response of sugarcane roots to methyl jasmonate

Bower, Neil I., Casu, Rosanne E., Maclean, Donald J., Reverter, Antonio, Chapman, Scott C. and Manners, John M. (2005) Transcriptional response of sugarcane roots to methyl jasmonate. Plant Science, 168 3: 761-772. doi:10.1016/j.plantsci.2004.10.006

Author Bower, Neil I.
Casu, Rosanne E.
Maclean, Donald J.
Reverter, Antonio
Chapman, Scott C.
Manners, John M.
Title Transcriptional response of sugarcane roots to methyl jasmonate
Journal name Plant Science   Check publisher's open access policy
ISSN 0168-9452
Publication date 2005
Sub-type Article (original research)
DOI 10.1016/j.plantsci.2004.10.006
Volume 168
Issue 3
Start page 761
End page 772
Total pages 12
Place of publication Shannon, Ireland
Publisher Elsevier
Collection year 2005
Language eng
Subject 270201 Gene Expression
770804 Control of pests and exotic species
Abstract The effect of methyl jasmonate treatment on gene expression in sugarcane roots signalling between roots and shoots was studied. A collection of 829 ESTs were obtained from sugarcane roots treated with the defence-regulator methyl jasmonate (MJ) treatment. A subset of 747 of these were combined with 4793 sugarcane ESTs obtained from stem tissues in a cDNA microarray and experiments undertaken to identify genes that were induced in roots 24-120 h following treatment with MJ. Two data analysis systems (t-statistic and tRMA) were used to analyse the microarray results and these methods identified a common set of 21 ESTs corresponding to transcripts significantly induced by MJ in roots and 23 that were reduced in expression following MJ treatment. The induction of six transcripts identified in the microarray analysis was tested and confirmed using northern blotting. Homologues of genes encoding lipoxygenase and PR-10 proteins were induced 824 It after MJ treatment while the other four selected transcripts were induced at later time points. Following treatment of roots with MJ, the lipoxygenase homologue, but not the PR-10 homologue, was induced in untreated stem and leaf tissues. The PR-10 homologue and a PR-1 homologue, but not the lipoxygenase homologue, were induced in untreated tissues after the application of SA to roots. Repeated foliar application of MJ had no apparent effects on plant growth and was demonstrated to increase lipoxygenase transcripts in roots, but did not increase transcript levels-of other genes tested. These results lay a foundation for further studies of induced pest and disease resistance in sugarcane roots. (C) 2004 Elsevier Ireland Ltd. All rights reserved.
Keyword Sugarcane
Methyl Jasmonate
Salicylic Acid
Biochemistry & Molecular Biology
Plant Sciences
Systemic Acquired-resistance
Transgenic Tobacco Plants
Microarray Analysis
Disease Resistance
Maturing Stem
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

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Created: Wed, 15 Aug 2007, 06:22:15 EST