The human stress-activated protein kinase-interacting 1 gene encodes JNK-binding proteins

Schroder, Wayne, Bushell, Gillian and Sculley, Tom (2005) The human stress-activated protein kinase-interacting 1 gene encodes JNK-binding proteins. Cellular Signalling, 17 6: 761-767. doi:10.1016/j.cellsig.2004.10.015

Author Schroder, Wayne
Bushell, Gillian
Sculley, Tom
Title The human stress-activated protein kinase-interacting 1 gene encodes JNK-binding proteins
Journal name Cellular Signalling   Check publisher's open access policy
ISSN 0898-6568
Publication date 2005
Sub-type Article (original research)
DOI 10.1016/j.cellsig.2004.10.015
Volume 17
Issue 6
Start page 761
End page 767
Total pages 7
Editor M. D. Houslay
Place of publication Philadelphia, PA, United States
Publisher Elsevier Science
Collection year 2005
Language eng
Subject 320299 Immunology not elsewhere classified
730299 Public health not elsewhere classified
Abstract The orthologous proteins of the stress-activated protein kinase-interacting 1 (Sin1) family have been implicated in several different signal transduction pathways. In this study, we have investigated the function of the full-length human Sin1 protein and a C-terminally truncated isoform, Sin 1 alpha, which is produced by alternative splicing. Immunoblot analysis using an anti-Sin 1 polyclonal antibody showed that full-length Sin I and several smaller isoforms are widely expressed. Sin 1 was demonstrated to bind to c-Jun N-terminal kinase (JNK) in vitro and in vivo, while no interaction with p38- or ERK1/2-family MAPKs was observed. The Sin1 alpha isoform could also form a complex with JNK in vivo. Despite localizing in distinct compartments within the cell, both Sin1 and Sin1 alpha co-localized with JNK, suggesting that the Sin1 proteins could recruit JNK. Over-expression of full-length Sin1 inhibited the activation of JNK by UV-C in DG75 cells, as well as basal JNK-activity in HEK293 cells. These data suggest that the human Sin1 proteins may act as scaffold molecules in the regulation of signaling by JNK. (c) 2004 Elsevier Inc. All rights reserved.
Keyword Sin 1
Scaffolding Protein
Cell Biology
N-terminal Kinase
Map Kinases
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2006 Higher Education Research Data Collection
School of Medicine Publications
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Citation counts: TR Web of Science Citation Count  Cited 22 times in Thomson Reuters Web of Science Article | Citations
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Created: Wed, 15 Aug 2007, 06:21:36 EST