Subcutaneous gas tensions closely track ileal mucosal gas tensions in a model of endotoxaemia without anaerobism

Venkatesh, Bala, Morgan, Thomas J., Hall, Jonathan, Endre, Zoltan and Willgoss, Desley (2005) Subcutaneous gas tensions closely track ileal mucosal gas tensions in a model of endotoxaemia without anaerobism. Intensive Care Medicine, 31 3: 447-453. doi:10.1007/s00134-005-2558-8


Author Venkatesh, Bala
Morgan, Thomas J.
Hall, Jonathan
Endre, Zoltan
Willgoss, Desley
Title Subcutaneous gas tensions closely track ileal mucosal gas tensions in a model of endotoxaemia without anaerobism
Journal name Intensive Care Medicine   Check publisher's open access policy
ISSN 0342-4642
1432-1238
Publication date 2005-03-01
Sub-type Article (original research)
DOI 10.1007/s00134-005-2558-8
Volume 31
Issue 3
Start page 447
End page 453
Total pages 7
Place of publication Heidelberg, Germany
Publisher Springer
Collection year 2005
Language eng
Subject C1
11 Medical and Health Sciences
Formatted abstract
Objective: Few comparative data exist on the responses of the subcutaneous and splanchnic circulations to evolving endotoxic shock. We therefore compared continuous subcutaneous pO2 (pO2sc) and pCO2 (pCO2sc) with simultaneous continuous gut luminal pCO2 (pCO2gi) in an animal model of endotoxaemia and examined whether changes in gas tensions track tissue energy charge (EC).
Design: Prospective observational study.
Subjects: Fourteen anaesthetized rats, 7 controls and 7 experimental.
Interventions: Controls were injected with saline, the experimental group with 20 mg/kg Klebsiella endotoxin. pCO2sc, pO2sc, and pCO2gi were measured continuously. Plasma lactate concentrations were measured at defined periods during the study. After 2 h ileal segments were snap frozen and assayed for tissue EC.
Measurements and results: Endotoxaemia resulted in a significant decrease in mean arterial blood pressure (132±9 to 71±20 mmHg) and pO2sc (71±23 to 33±22 torr) and a significant increase in pCO2gi (58±10 to 90±20 torr) and pCO2sc (56±6 to 81±25 torr). During endotoxaemia pCO2gi was directly correlated with pCO2sc (R 2=0.5) and inversely correlated with pO2sc (R 2=0.63). Plasma lactate concentrations were significantly elevated from baseline in the endotoxin limb. The mean EC was not significantly different in the two groups.
Conclusions: Both subcutaneous tissue gas tensions and intestinal luminal carbon dioxide tensions are rapidly responsive during evolving hypodynamic endotoxic shock. Alterations in tissue gas tensions were not associated with dysoxia.
Keyword Mucosal
Subcutaneous
Oxygen
Carbon Dioxide
Endotoxaemia
Dysoxia
Critical Care Medicine
Acid-base
Gastrointestinal-tract
Hemorrhagic-shock
Oxygen-tensions
Intramucosal Ph
Cardiac-output
Gut
Ischemia
Sepsis
Saline
Q-Index Code C1
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
2006 Higher Education Research Data Collection
School of Medicine Publications
 
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Created: Wed, 15 Aug 2007, 16:21:29 EST