Rheumatoid arthritis synovium contains plasmacytoid dendritic cells

Cavanagh, Lois L., Boyce, Amanda, Smith, Louise, Padmanabha, Jagadish, Filgueira, Luis, Pietschmann, Peter and Thomas, Ranjeny (2005) Rheumatoid arthritis synovium contains plasmacytoid dendritic cells. Arthritis Research and Therapy, 7 2: R230-R240. doi:10.1186/ar1467

Author Cavanagh, Lois L.
Boyce, Amanda
Smith, Louise
Padmanabha, Jagadish
Filgueira, Luis
Pietschmann, Peter
Thomas, Ranjeny
Title Rheumatoid arthritis synovium contains plasmacytoid dendritic cells
Journal name Arthritis Research and Therapy   Check publisher's open access policy
ISSN 1465-9905
Publication date 2005-01-11
Sub-type Article (original research)
DOI 10.1186/ar1467
Open Access Status DOI
Volume 7
Issue 2
Start page R230
End page R240
Total pages 11
Place of publication London, United Kingdom
Publisher Biomed Central
Collection year 2005
Language eng
Formatted abstract
We have previously described enrichment of antigen-presenting HLA-DR+ nuclear RelB+ dendritic cells (DCs) in rheumatoid arthritis (RA) synovium. CD123+ HLA- DR+ plasmacytoid DCs (pDCs) and their precursors have been identified in human peripheral blood (PB), lymphoid tissue, and some inflamed tissues. We hypothesized recruitment of pDCs into the inflamed RA synovial environment and their contribution as antigen-presenting cells (APCs) and inflammatory cells in RA. CD11c+ myeloid DCs and CD123+ pDCs were compared in normal and RA PB, synovial fluid ( SF), and synovial tissue by flow cytometry, immunohistochemistry, and electron microscopy and were sorted for functional studies. Nuclear RelB-CD123+ DCs were located in perivascular regions of RA, in a similar frequency to nuclear RelB+ CD123- DCs, but not normal synovial tissue sublining. Apart from higher expression of HLA-DR, the numbers and phenotypes of SF pDCs were similar to those of normal PB pDCs. While the APC function of PB pDCs was less efficient than that of PB myeloid DCs, RA SF pDCs efficiently activated resting allogeneic PB T cells, and high levels of IFN-gamma, IL-10, and tumor necrosis factor alpha were produced in response to incubation of allogeneic T cells with either type of SF DCs. Thus, pDCs are recruited to RA synovial tissue and comprise an APC population distinct from the previously described nuclear RelB+ synovial DCs. pDCs may contribute significantly to the local inflammatory environment.
Keyword Dendritic cells
Rheumatoid arthritis
Inflammatory arthritis
Adhesion molecules
Cd40 ligand
Lymphoid neogenesis
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

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Created: Wed, 15 Aug 2007, 06:20:59 EST