Response of crayfish, Procambarus clarkii, haemocytes infected by white spot syndrome virus

Shi, Z., Wang, H., Zhang, J., Xie, Y., Li, L., Chen, X., Edgerton, B. F. and Bonami, J. R. (2005) Response of crayfish, Procambarus clarkii, haemocytes infected by white spot syndrome virus. Journal of Fish Diseases, 28 3: 151-156.

Document type: Journal Article
Sub-type: Article
Collections: 2006 Higher Education Research Data Collection
Centre for Marine Studies Publications

Author Shi, Z.
Wang, H.
Zhang, J.
Xie, Y.
Li, L.
Chen, X.
Edgerton, B. F.
Bonami, J. R.
Title Response of crayfish, Procambarus clarkii, haemocytes infected by white spot syndrome virus
Journal name Journal of Fish Diseases  (ERA 2012 Listed)    (ERA 2010 Rank B)
Publication date 2005-03
Sub-type Article
Volume number 28
Issue number 3
ISSN 0140-7775
Start page 151
End page 156
Total pages 6
Place of publication Oxford
Publisher Blackwell Publishing Ltd
Collection year 2005
Language eng
Subject C1
Abstract White spot syndrome virus ( WSSV) is a serious pathogen of aquatic crustaceans. Little is known about its transmission in vivo and the immune reaction of its hosts. In this study, the circulating haemocytes of crayfish, Procambarus clarkii, infected by WSSV, and primary haemocyte cultures inoculated with WSSV, were collected and observed by transmission electron microscopy and light microscopy following in situ hybridization. In ultrathin sections of infected haemocytes, the enveloped virions were seen to be phagocytosed in the cytoplasm and no viral particles were observed in the nuclei. In situ hybridization with WSSV-specific probes also demonstrated that there were no specific positive signals present in the haemocytes. Conversely, strong specific positive signals showed that WSSV replicated in the nuclei of gill cells. As a control, the lymphoid organ of shrimp, Penaeus monodon, infected by WSSV was examined by in situ hybridization which showed that WSSV did not replicate within the tubules of the lymphoid organ. In contrast to previous studies, it is concluded that neither shrimp nor crayfish haemocytes support WSSV replication.White spot syndrome virus (WSSV) is a serious pathogen of aquatic crustaceans. Little is known about its transmission in vivo and the immune reaction of its hosts. In this study, the circulating haemocytes of crayfish, Procambarus clarkii, infected by WSSV, and primary haemocyte cultures inoculated with WSSV, were collected and observed by transmission electron microscopy and light microscopy following in situ hybridization. In ultra-thin sections of infected haemocytes, the enveloped virions were seen to be phagocytosed in the cytoplasm and no viral particles were observed in the nuclei. In situ hybridization with WSSV-specific probes also demonstrated that there were no specific positive signals present in the haemocytes. Conversely, strong specific positive signals showed that WSSV replicated in the nuclei of gill cells. As a control, the lymphoid organ of shrimp, Penaeus monodon, infected by WSSV was examined by in situ hybridization which showed that WSSV did not replicate within the tubules of the lymphoid organ. In contrast to previous studies, it is concluded that neither shrimp nor crayfish haemocytes support WSSV replication.
Keyword Crayfish
Electron Microscopy
Haemocyte Response
In Situ Hybridization
Procambarus Clarkii
White Spot Syndrome Virus
Fisheries
Marine & Freshwater Biology
Veterinary Sciences
Syndrome Baculovirus Wsbv
Shrimp Penaeus-monodon
Genome Sequence
Ultrastructure
System
Tissue
Cells
Gene
Wssv
Q-Index Code C1
 
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