Rab11 in recycling endosomes regulates the sorting and basolateral transport of E-cadherin

Lock, John G. and Stow, Jennifer L. (2005) Rab11 in recycling endosomes regulates the sorting and basolateral transport of E-cadherin. Molecular Biology of The Cell, 16 4: 1744-1755. doi:10.1091/mbc.E04-10-0867

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Author Lock, John G.
Stow, Jennifer L.
Title Rab11 in recycling endosomes regulates the sorting and basolateral transport of E-cadherin
Journal name Molecular Biology of The Cell   Check publisher's open access policy
ISSN 1059-1524
1939-4586
Publication date 2005
Sub-type Article (original research)
DOI 10.1091/mbc.E04-10-0867
Open Access Status File (Publisher version)
Volume 16
Issue 4
Start page 1744
End page 1755
Total pages 12
Place of publication Bethesda
Publisher American Society for Cell Biology
Collection year 2005
Language eng
Subject C1
270103 Protein Targeting and Signal Transduction
780106 Political science and public policy
Abstract E-cadherin plays an essential role in cell polarity and cell-cell adhesion; however, the pathway for delivery of E-cadherin to the basolateral membrane of epithelial cells has not been fully characterized. We first traced the post-Golgi, exocytic transport of GFP-tagged E-cadherin (Ecad-GFP) in unpolarized cells. In live cells, Ecad-GFP was found to exit the Golgi complex in pleiomorphic tubulovesicular carriers, which, instead of moving directly to the cell surface, most frequently fused with an intermediate compartment, subsequently identified as a Rab11-positive recycling endosome. In MDCK cells, basolateral targeting of E-cadherin relies on a dileucine motif. Both E-cadherin and a targeting mutant, Delta S1-E-cadherin, colocalized with Rab11 and fused with the recycling endosome before diverging to basolateral or apical membranes, respectively. In polarized and unpolarized cells, coexpression of Rab11 mutants disrupted the cell surface delivery of E-cadherin and caused its mistargeting to the apical membrane, whereas apical Delta S1-E-cadherin was unaffected. We thus demonstrate a novel pathway for Rab11 dependent, dileucine-mediated, mu 1B-independent sorting and basolateral trafficking, exemplified by E-cadherin. The recycling endosome is identified as an intermediate compartment for the post-Golgi trafficking and exocytosis of E-cadherin, with a potentially important role in establishing and maintaining cadherin-based adhesion.
Keyword Cell Biology
Darby Canine Kidney
Plasma-membrane Transport
Basal-lateral Membrane
Golgi Network
Cell-surface
Epithelial-cells
Living Cells
Subapical Compartment
Parietal-cells
Mdck Cells
Q-Index Code C1

 
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Created: Wed, 15 Aug 2007, 06:18:05 EST