Cyclosporine A induced changes to plasma and erythrocyte antioxidant defences

Fletcher, L. A., Fassett, R. G., Richards, R. S. and Coombes, J. S. (2005) Cyclosporine A induced changes to plasma and erythrocyte antioxidant defences. Redox Report, 10 1: 2-8. doi:10.1179/135100005X21543

Author Fletcher, L. A.
Fassett, R. G.
Richards, R. S.
Coombes, J. S.
Title Cyclosporine A induced changes to plasma and erythrocyte antioxidant defences
Journal name Redox Report   Check publisher's open access policy
ISSN 1351-0002
Publication date 2005
Sub-type Article (original research)
DOI 10.1179/135100005X21543
Volume 10
Issue 1
Start page 2
End page 8
Total pages 7
Editor N. Hunt
Place of publication Leeds
Publisher Maney Publishing
Collection year 2005
Language eng
Subject C1
730106 Cardiovascular system and diseases
321003 Cardiology (incl. Cardiovascular Diseases)
Abstract Organ transplant recipients develop pronounced cardiovascular disease, and decreased antioxidant capacity in plasma and erythrocytes is associated with the pathogenesis of this disease. These experiments tested the hypothesis that the immunosuppressant cyclosporine A (CsA) alters erythrocyte redox balance and reduces plasma antioxidant capacity. Female Sprague-Dawley rats were randomly assigned to a control or CsA treated group. Treatment animals received 25 mg/kg/day of CsA via intraperitoneal injection for 18 days. Control rats were injected with the same volume of the vehicle. Three hours after the final CsA injection, rats were exsanguinated and plasma analysed for total antioxidant status (TAS), alpha-tocopherol, malondialdehyde (MDA), and creatinine. Erythrocytes were analysed for superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX) and glucose-6-phosphate dehydrogenase (G6PD) activities, alpha-tocopherol, and MDA. CsA administration resulted in a significant (P < 0.05) decrease in plasma TAS and significant increases (P < 0.05) in plasma creatinine and MDA. Erythrocyte CAT was significantly (P < 0.05) increased in CsA treated rats compared to controls. There were no significant differences (P > 0.05) in erythrocyte SOD, GPX, G6PD, alpha-tocopherol or MDA between groups. In summary, CsA alters erythrocyte antioxidant defence and decreases plasma total antioxidant capacity.
Keyword Biochemistry & Molecular Biology
Cyclosporine A
Reactive Oxygen Species
Free Radicals
Renal-transplant Patients
Glutathione-peroxidase Activity
Oxygen Free-radicals
Oxidative Stress
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

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Created: Wed, 15 Aug 2007, 06:17:08 EST