Macrophages overexpressing tartrate-resistant acid phosphatase show altered profile of free radical production and enhanced capacity of bacterial killing

Raisanen, S. R., Alatalo, S. L., Ylipahkala, H., Halleen, J. M., Cassady, A. I., Hume, D. A. and Vaananen, H. K. (2005) Macrophages overexpressing tartrate-resistant acid phosphatase show altered profile of free radical production and enhanced capacity of bacterial killing. Biochemical And Biophysical Research Communications, 331 1: 120-126. doi:10.1016/j.bbrc.2005.03.133


Author Raisanen, S. R.
Alatalo, S. L.
Ylipahkala, H.
Halleen, J. M.
Cassady, A. I.
Hume, D. A.
Vaananen, H. K.
Title Macrophages overexpressing tartrate-resistant acid phosphatase show altered profile of free radical production and enhanced capacity of bacterial killing
Journal name Biochemical And Biophysical Research Communications   Check publisher's open access policy
ISSN 0006-291X
Publication date 2005
Sub-type Article (original research)
DOI 10.1016/j.bbrc.2005.03.133
Volume 331
Issue 1
Start page 120
End page 126
Total pages 7
Place of publication San Diego, CA, United States
Publisher Academic Press Inc Elsevier Science
Collection year 2005
Language eng
Subject 320202 Cellular Immunology
730102 Immune system and allergy
Abstract Activated macrophages and osteoclasts express high amounts of tartrate-resistant acid phosphatase (TRACP, acp5). TRACP has a binuclear iron center with a redox-active iron that has been shown to catalyze the formation of reactive oxygen species (ROS) by Fenton's reaction. Previous Studies Suggest that ROS generated by TRACP may participate in degradation of endocytosed bone matrix products in resorbing osteoclasts and degradation of foreign Compounds during. antigen presentation in activated macrophages. Here we have compared free radical production in macrophages of TRACP overexpressing (TRACP +) and wild-type (WT) mice. TRACP overexpression increased both ROS levels and Superoxide production. Nitric oxide production was increased in activated macrophages or WT mice, but not in TRACP+ mice, Macrophages from TRACP+ mice showed increased capacity or bacterial killing. Recombinant TRACP enzyme was capable of bacterial killing in the presence of hydrogen peroxide. These results suggest that TRACP has an important biological function in immune defense systern.
Keyword Antigen Presentation
Immune Defense
Phagocytosis
Nadph Oxidase
Nos
Biochemistry & Molecular Biology
Biophysics
Nf-kappa-b
Bone-resorption Products
Nitric-oxide Production
Staphylococcus-aureus
Alveolar Macrophages
Hydrogen-peroxide
Reactive Oxygen
Expression
Osteoclast
Activation
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

 
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Created: Wed, 15 Aug 2007, 06:13:19 EST