The BeWo choriocarcinoma cell line as a model of iodide transport by placenta

Manley, S. W., Li, H. and Mortimer, R. H. (2005) The BeWo choriocarcinoma cell line as a model of iodide transport by placenta. Placenta, 26 5: 380-386. doi:10.1016/j.placenta.2004.07.004

Author Manley, S. W.
Li, H.
Mortimer, R. H.
Title The BeWo choriocarcinoma cell line as a model of iodide transport by placenta
Journal name Placenta   Check publisher's open access policy
ISSN 0143-4004
Publication date 2005
Sub-type Article (original research)
DOI 10.1016/j.placenta.2004.07.004
Volume 26
Issue 5
Start page 380
End page 386
Total pages 7
Place of publication Oxford, United Kingdom
Publisher Elsevier
Collection year 2005
Language eng
Subject 1103 Clinical Sciences
Abstract Cultured human choriocarcinoma cells of the BeWo line exhibited saturable accumulation of radioiodide. Inhibition by competing anions followed the affinity series perchlorate >= iodide >= thiocyanate, consistent with uptake through the thyroid iodide transporter, NIS, whose messenger RNA was found in BeWo cells, and whose protein was distributed towards the apical pole of the cells. Efflux obeyed first order kinetics and was inhibited by DIDS, an antagonist of anion exchangers including pendrin, whose messenger RNA was also present. In cultures where iodide uptake through NIS was blocked with excess perchlorate, radiolodide accumulation was stimulated by exposure to medium in which physiological anions were replaced by 2-morpholinoethanesulfonic acid (MES), consistent with the operation of an anion exchange mechanism taking up iodide. Chloride in the medium was more effective than sulfate at inhibiting this uptake, matching the ionic specificity of pendrin. These studies provide evidence that the trophoblast accumulates iodide through NIS and releases it to the fetal compartment through pendrin. (c) 2004 Elsevier Ltd. All rights reserved.
Keyword Developmental Biology
Obstetrics & Gynecology
Reproductive Biology
Pendred Syndrome
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
2006 Higher Education Research Data Collection
School of Medicine Publications
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Citation counts: TR Web of Science Citation Count  Cited 18 times in Thomson Reuters Web of Science Article | Citations
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Created: Wed, 15 Aug 2007, 06:10:51 EST