Noninvasive localization of petroleum-derived spray oil in plants with chemical shift selective magnetic resonance imaging

Tan, B. L., Sarafis, V., Beattie, G. A. C. and Spooner-Hart, R. (2005) Noninvasive localization of petroleum-derived spray oil in plants with chemical shift selective magnetic resonance imaging. Hortscience, 40 3: 720-725.

Author Tan, B. L.
Sarafis, V.
Beattie, G. A. C.
Spooner-Hart, R.
Title Noninvasive localization of petroleum-derived spray oil in plants with chemical shift selective magnetic resonance imaging
Journal name Hortscience   Check publisher's open access policy
ISSN 0018-5345
Publication date 2005
Sub-type Article (original research)
Volume 40
Issue 3
Start page 720
End page 725
Total pages 6
Editor LeRon Rbbins
Place of publication Alexandria, VA, United States
Publisher American Society for Horticultural Science
Collection year 2005
Language eng
Subject C1
270504 Invertebrate Biology
780105 Biological sciences
Abstract Nuclear magnetic resonance (NMR) spectroscopy and magnetic resonance imaging (MRI) were used to detect petroleum-derived spray oils (PDSOs) in citrus seedlings and trees. The NMR spectrum of the phantom containing 10% (v/v) of a nC24 agricultural mineral oil (AMO) showed the resonance of the water protons at delta = 5 ppm, while the resonance of the oil protons at delta = 1.3 to 1.7 ppm. The peak resolution and the chemical shift difference of more than 3.3 ppm between water and oil protons effectively differentiated water and the oil. Chemical shift selective imaging (CSSI) was performed to localize the AMO within the stems of Citrus trifoliata L. seedlings after the application of a 4% (v/v) spray. The chemical shift selective images of the oil were acquired by excitation at delta = 1.5 ppm by averaging over 400 transients in each phase-encoding step. Oil was mainly detected in the outer cortex of stems within 10 d of spray application; some oil was also observed in the inner vascular bundle and pith of the stems at this point. CSSI was also applied to investigate the persistence of oil deposits in sprayed mature Washington navel orange (Citrus x aurantium L.) trees in an orchard. The trees were treated with either fourteen 0.25%, fourteen 0.5%, four 1.75%, or single 7% sprays of a nC23 horticultural mineral oil (HMO) 12 to 16 months before examination of plant tissues by CSSI, and were still showing symptoms of chronic phytotoxicity largely manifested as reduced yield. The oil deposits were detected in stems of sprayed flushes and unsprayed flushes produced 4 to 5 months after the last spray was applied, suggesting a potential movement of the oil via phloem and a correlation of the persistence of oil deposit in plants and the phytotoxicity. The results demonstrate that MRI is an effective method to probe the uptake and localization of PDSOs and other xenobiotics in vivo in plants noninvasively and nondestructively.
Keyword petroleum-derived spray oil
localization
NMR spectroscopy
MRI
chemical shift selective image
Citrus
Histochemistry
Microscopy
Nmr
Penetration
Deposits
Leaves
Roots
Trees
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2006 Higher Education Research Data Collection
School of Physical Sciences Publications
 
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Created: Wed, 15 Aug 2007, 06:09:11 EST