Co-expression of SOX9 and SOX10 during melanocytic differentiation in vitro

Cook, A. L., Smith, A. G., Smit, D. J., Leonard, J. H. and Sturm, R. A. (2005) Co-expression of SOX9 and SOX10 during melanocytic differentiation in vitro. Experimental Cell Research, 308 1: 222-235.


Author(s) Cook, A. L.
Smith, A. G.
Smit, D. J.
Leonard, J. H.
Sturm, R. A.
Title Co-expression of SOX9 and SOX10 during melanocytic differentiation in vitro
Journal name Experimental Cell Research
Publication date 2005
Sub-type Article
Volume number 308
Issue number 1
ISSN 0014-4827
Start page 222
End page 235
Total pages 14
Place of publication San Diego
Publisher Elsevier Inc
Collection year 2005
Subject C1
270106 Cell Development (incl. Cell Division and Apoptosis)
730117 Skin and related disorders
Abstract Investigations into pigment cell biology have relied on the ability to culture both murine and human melanocytes, numerous melanoma cell lines and more recently, murine and human melanoblasts. Melanoblast culture requires medium supplemented with a range of growth factors including Stem Cell Factor, Endothelin-3 and Fibroblast Growth Factor-2, withdrawal of which causes the cells to differentiate into melanocytes. Using the human melanoblast culture system, we have now examined the expression and/or DNA binding activity of several transcription factors implicated in melanocytic development and differentiation. Of these, the POU domain factor BRN2 and the SOX family member SOX10 are both highly expressed in unpigmented melanocyte precursors but are down-regulated upon differentiation. In contrast, the expression levels of the previously described MITF and PAX3 transcription factors remain relatively constant during the melanoblast-melanocyte transition. Moreover, BRN2 ablated melanoma cells lack expression of SOX10 and MITF but retain PAX3. A novel finding implicates a second SOX protein, SOX9, as a potential melanogenic transcriptional regulator, as its expression level is increased following the down-regulation of BRN2 and SOX10 in differentiated melanoblasts. Our results suggest that a complex network of transcription factor interactions requiring proper temporal coordination is necessary for acquisition and maintenance of the melanocytic phenotype. (c) 2005 Elsevier Inc. All rights reserved.
Keyword(s) Pou
Sox
Pax
Mitf
Ednrb
Differentiation
Melanoma
Oncology
Cell Biology
Octamer-binding-protein
Transcription Factor
Waardenburg-syndrome
Neural Crest
Pou Proteins
Melanoma Proliferation
Hirschsprung-disease
Mouse Model
Gene
Expression
 
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