Investigation of ansB and sspA derived promoters for multi- and single-copy antigen expression in attenuated Salmonella enterica var. typhimurium

Terry, Tasmin D., Downes, John E., Dowideit, Stefanie J., Mabbett, Amanda N. and Jennings, Michael P. (2005) Investigation of ansB and sspA derived promoters for multi- and single-copy antigen expression in attenuated Salmonella enterica var. typhimurium. Vaccine, 23 36: 4521-4531. doi:10.1016/j.vaccine.2005.04.028


Author Terry, Tasmin D.
Downes, John E.
Dowideit, Stefanie J.
Mabbett, Amanda N.
Jennings, Michael P.
Title Investigation of ansB and sspA derived promoters for multi- and single-copy antigen expression in attenuated Salmonella enterica var. typhimurium
Formatted title
Investigation of ansB and sspA derived promoters for multi- and single-copy antigen expression in attenuated Salmonella enterica var. typhimurium
Journal name Vaccine   Check publisher's open access policy
ISSN 0264-410X
1873-2518
Publication date 2005
Sub-type Article (original research)
DOI 10.1016/j.vaccine.2005.04.028
Volume 23
Issue 36
Start page 4521
End page 4531
Total pages 11
Place of publication Oxford, United Kingdom
Publisher Elsevier
Collection year 2005
Language eng
Subject C1
270306 Microbial Genetics
730101 Infectious diseases
Abstract Five candidate promoters were examined to determine their utility in directing immunogenic levels of expression of the C fragment from tetanus toxin in attenuated S. enterica used as an oral vaccine in mice. Promoters derived from the genes encoding the stringent starvation protein (sspA) from E. coli and S. enterica, but not ansB derived promoters, expressed immunogenic levels of C fragment from multi-copy plasmids in attenuated S. enterica in vivo and, following oral immunization, induced high titre specific anti-tetanus toxoid serum antibodies. We also demonstrate that not only the choice of promoter, replicon and growth conditions but also how expression constructs are assembled in the chosen plasmid is critical for the successful development of plasmid-based antigen delivery systems using attenuated S. enterica. In addition, the S. enterica sspA promoter is able to elicit anti-tetanus toxoid antibodies in mice when the psspA-tetC expression cassette is integrated in single copy on the S. enterica chromosome.
Keyword Live Attenuated Salmonella Typhimurium
Oral Vaccine Delivery
Starvation Induced Promoters
Immunology
Medicine, Research & Experimental
Veterinary Sciences
Stringent Starvation Protein
Escherichia-coli
Null Mutation
Aroa-arod
Vaccine
Construction
Strains
Gene
Chickens
Plasmids
Q-Index Code C1
Institutional Status UQ

 
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Created: Wed, 15 Aug 2007, 06:01:08 EST