In vitro and in silico analysis of signal peptides from the human blood fluke, Schistosoma mansoni

Pearson, M. S., McManus, D. P., Smyth, D. J., Lewis, F. A. and Loukas, A. (2005) In vitro and in silico analysis of signal peptides from the human blood fluke, Schistosoma mansoni. Fems Immunology And Medical Microbiology, 45 2: 201-211. doi:10.1016/j.femsim.2005.03.009

Author Pearson, M. S.
McManus, D. P.
Smyth, D. J.
Lewis, F. A.
Loukas, A.
Title In vitro and in silico analysis of signal peptides from the human blood fluke, Schistosoma mansoni
Journal name Fems Immunology And Medical Microbiology   Check publisher's open access policy
ISSN 0928-8244
Publication date 2005
Sub-type Article (original research)
DOI 10.1016/j.femsim.2005.03.009
Volume 45
Issue 2
Start page 201
End page 211
Total pages 11
Place of publication Amsterdam
Publisher Elsevier Science Bv
Collection year 2005
Language eng
Abstract Proteins secreted by and anchored on the surfaces of parasites are in intimate contact with host tissues. The transcriptome of infective cercariae of the blood fluke, Schistosoma mansoni, was screened using signal sequence trap to isolate cDNAs encoding predicted proteins with an N-terminal signal peptide. Twenty cDNA fragments were identified, most of which contained predicted signal peptides or transmembrane regions, including a novel putative seven-transmembrane receptor and a membrane-associated mitogen-activated protein kinase. The developmental expression pattern within different life-cycle stages ranged from ubiquitous to a transcript that was highly upregulated in the cercaria. A bioinformatics-based comparison of 100 signal peptides from each of schistosomes, humans, a parasitic nematode and Escherichia coli showed that differences in the sequence composition of signal peptides, notably the residues flanking the predicted cleavage site, might account for the negative bias exhibited in the processing of schistosome signal peptides in mammalian cells. (c) 2005 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.
Keyword Schistosoma Mansoni
Signal Peptide
Signal Sequence Trap
Infectious Diseases
Protein-coupled Receptor
Sequence Trap Method
Secreted Proteins
Cleavage Sites
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
2006 Higher Education Research Data Collection
School of Medicine Publications
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Citation counts: TR Web of Science Citation Count  Cited 7 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 9 times in Scopus Article | Citations
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Created: Wed, 15 Aug 2007, 05:58:29 EST