Cardiovascular actions of the venom from the Irukandji (Carukia barnesi) jellyfish: Effects in human, rat and guinea-pig tissues in vitro and in pigs in vivo

Winkel, KD, Tibballs, J, Molenaar, P, Lambert, G, Coles, P, Ross-Smith, M, Wiltshire, C, Fenner, PJ, Gershwin, LA, Hawdon, GM, Wright, CE and Angus, JA (2005) Cardiovascular actions of the venom from the Irukandji (Carukia barnesi) jellyfish: Effects in human, rat and guinea-pig tissues in vitro and in pigs in vivo. Clinical And Experimental Pharmacology And Physiology, 32 9: 777-788. doi:10.1111/j.1440-1681.2005.04258.x


Author Winkel, KD
Tibballs, J
Molenaar, P
Lambert, G
Coles, P
Ross-Smith, M
Wiltshire, C
Fenner, PJ
Gershwin, LA
Hawdon, GM
Wright, CE
Angus, JA
Title Cardiovascular actions of the venom from the Irukandji (Carukia barnesi) jellyfish: Effects in human, rat and guinea-pig tissues in vitro and in pigs in vivo
Journal name Clinical And Experimental Pharmacology And Physiology   Check publisher's open access policy
ISSN 0305-1870
Publication date 2005
Sub-type Article (original research)
DOI 10.1111/j.1440-1681.2005.04258.x
Volume 32
Issue 9
Start page 777
End page 788
Total pages 12
Editor W. P. Anderson
Place of publication Carlton South, Australia
Publisher Blackwell Publishing Asia
Collection year 2005
Language eng
Subject C1
321003 Cardiology (incl. Cardiovascular Diseases)
730106 Cardiovascular system and diseases
Abstract 1. We have investigated the cardiovascular pharmacology of the crude venom extract (CVE) from the potentially lethal, very small carybdeid jellyfish Carukia barnesi, in rat, guinea-pig and human isolated tissues and anaesthetized piglets. 2. In rat and guinea-pig isolated right atria, CVE (0.1-10 mu g/mL) caused tachycardia in the presence of atropine (I mu mol/L), a response almost completely abolished by pretreatment with tetrodotoxin (TTX; 0.1 mu mol/L). In paced left atria from guinea-pig or rat, CVE (0.1-3 mu g/mL) caused a positive inotropic response in the presence of atropine (1 mu mol/L). 3. In rat mesenteric small arteries, CVE (0.1-30 mu g/mL) caused concentration-dependent contractions that were unaffected by 0.1 mu mol/L TTX, 0.3 mu mol/L prazosin or 0.1 mu mol/L co-conotoxin GVIA. 4. Neither the rat right atria tachycardic response nor the contraction of rat mesenteric arteries to CVE were affected by the presence of box jellyfish (Chironex fleckeri) antivenom (92.6 units/mL). 5. In human isolated driven right atrial trabeculae muscle strips, CVE (10 mu g/mL) tended to cause an initial fall, followed by a more sustained increase, in contractile force. In the presence of atropine (I mu mol/L), CVE only caused a positive inotropic response. In separate experiments in the, presence of propranolol (0.2 mu mol/L), the negative inotropic effect of CVE was enhanced, whereas the positive inotropic response was markedly decreased. 6. In anaesthetized piglets, CVE (67 mu g/kg, i.v.) caused sustained tachycardia and systemic and pulmonary hypertension. Venous blood samples demonstrated a marked elevation in circulating levels of noradrenaline and adrenaline. 7. We conclude that C. barnesi venom may contain a neural sodium channel activator (blocked by TTX) that, in isolated atrial tissue (and in vivo), causes the release of transmitter (and circulating) catecholamines. The venom may also contain a 'direct' vasoconstrictor component. These observations explain, at least in part, the clinical features of the potentially deadly Irukandji syndrome.
Keyword Carukia Barnesi
Catecholamines
Irukandji Syndrome
Jellyfish
Neuronal Sodium Channel
Noradrenaline
Sympathetic Nervous System
Venom
Pharmacology & Pharmacy
Physiology
Cubomedusan Chironex-fleckeri
Scorpion Envenomation
Clinical Implications
Partial-purification
Magnesium-sulfate
Nematocyst Venom
Toxic Material
Heart-failure
Sea Wasp
Beta(1)-adrenoceptor
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
2006 Higher Education Research Data Collection
School of Medicine Publications
 
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