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AAA ATPases regulate membrane association of yeast oxysterol binding proteins and sterol metabolism
Wang, PH, Zhang, Y, Li, HZ, Chieu, HK, Munn, AL and Yang, HY (2005) AAA ATPases regulate membrane association of yeast oxysterol binding proteins and sterol metabolism. Embo Journal, 24 17: 2989-2999.
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| Author(s) |
Wang, PH
Zhang, Y
Li, HZ
Chieu, HK
Munn, AL
Yang, HY
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| Title |
AAA ATPases regulate membrane association of yeast oxysterol binding proteins and sterol metabolism
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| Journal name |
Embo Journal
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| Publication date |
2005
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| Volume number |
24
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| Issue number |
17
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| ISBN |
0261-4189
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| Start page |
2989
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| End page |
2999
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| Total pages |
11
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| Place of publication |
New York
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| Publisher |
Nature Publishing Group
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| Collection year |
2005
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| Subject |
C1
780105 Biological sciences
270103 Protein Targeting and Signal Transduction
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| Abstract |
The yeast genome encodes seven oxysterol binding protein homologs, Osh1p-Osh7p, which have been implicated in regulating intracellular lipid and vesicular transport. Here, we show that both Osh6p and Osh7p interact with Vps4p, a member of the AAA ( ATPases associated with a variety of cellular activities) family. The coiled-coil domain of Osh7p was found to interact with Vps4p in a yeast two-hybrid screen and the interaction between Osh7p and Vps4p appears to be regulated by ergosterol. Deletion of VPS4 induced a dramatic increase in the membrane-associated pools of Osh6p and Osh7p and also caused a decrease in sterol esterification, which was suppressed by overexpression of OSH7. Lastly, overexpression of the coiled-coil domain of Osh7p (Osh7pCC) resulted in a multi-vesicular body sorting defect, suggesting a dominant negative role of Osh7pCC possibly through inhibiting Vps4p function. Our data suggest that a common mechanism may exist for AAA proteins to regulate the membrane association of yeast OSBP proteins and that these two protein families may function together to control subcellular lipid transport.
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| Keyword(s) |
Aaa Atpase
Lipid Transport
Oxysterol Binding Protein (osbp)
Biochemistry & Molecular Biology
Cell Biology
Hamster Ovary Cells
Pleckstrin Homology Domain
Saccharomyces-cerevisiae
Multivesicular-body
Golgi-apparatus
Endoplasmic-reticulum
Vps Protein
Transport
Complex
Cholesterol
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