Localization and movement of mineral oil in plants by fluorescence and confocal microscopy

Tan, B. L., Sarafis, V., Beattie, G. A. C., White, R., Darley, E. M. and Spooner-Hart, R. (2005) Localization and movement of mineral oil in plants by fluorescence and confocal microscopy. Journal of Experimental Botany, 56 420: 2755-2763. doi:10.1093/jxb/eri269


Author Tan, B. L.
Sarafis, V.
Beattie, G. A. C.
White, R.
Darley, E. M.
Spooner-Hart, R.
Title Localization and movement of mineral oil in plants by fluorescence and confocal microscopy
Journal name Journal of Experimental Botany   Check publisher's open access policy
ISSN 0022-0957
1460-2431
Publication date 2005-10
Sub-type Article (original research)
DOI 10.1093/jxb/eri269
Volume 56
Issue 420
Start page 2755
End page 2763
Total pages 9
Place of publication Oxford, United Kingdom
Publisher Oxford University Press
Collection year 2005
Language eng
Subject 06 Biological Sciences
Abstract Fluorescence and confocal laser scanning microscopy were explored to investigate the movement and localization of mineral oils in citrus. In a laboratory experiment, fluorescence microscopy observation indicated that when a 'narrow' distillation fraction of an nC23 horticultural mineral oil was applied to adaxial and opposing abaxial leaf surfaces of potted orange [Citrus x aurantium L. (Sapindales: Rutaceae)] trees, oil penetrated steadily into treated leaves and, subsequently, moved to untreated petioles of the leaves and adjacent untreated stems. In another experiment, confocal laser scanning microscopy was used to visualize the penetration into, and the subsequent cellular distribution of, an nC24 agricultural mineral oil in C. trifoliata L. seedlings. Oil droplets penetrated or diffused into plants via both stomata and the cuticle of leaves and stems, and then moved within intercellular spaces and into various cells including phloem and xylem. Oil accumulated in droplets in intercellular spaces and within cells near the cell membrane. Oil entered cells without visibly damaging membranes or causing cell death. In a field experiment with mature orange trees, droplets of an nC23 horticultural mineral oil were observed, by fluorescence microscopy, in phloem sieve elements in spring flush growth produced 4-5 months and 16-17 months after the trees were sprayed with oil. These results suggest that movement of mineral oil in plants is both apoplastic via intercellular spaces and symplastic via plasmodesmata. The putative pattern of the translocation of mineral oil in plants and its relevance to oil-induced chronic phytotoxicity are discussed.
Keyword Apoplast
Confocal Laser Scanning Microscopy
Fluorescence Microscopy
Localization
Mineral Oil
Penetration
Phloem
Symplast
Translocation
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

 
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