Myosin 2 is a key Rho kinase target necessary for the local concentration of E-cadherin at cell-cell contacts

Shewan, A. M., Maddugoda, M., Kraemer, A., Stehbens, S. J., Verma, S., Kovacs, E. M. and Yap, A. S. (2005) Myosin 2 is a key Rho kinase target necessary for the local concentration of E-cadherin at cell-cell contacts. Molecular Biology of The Cell, 16 10: 4531-4542. doi:10.1091/mbc.E05-04-0330

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Author Shewan, A. M.
Maddugoda, M.
Kraemer, A.
Stehbens, S. J.
Verma, S.
Kovacs, E. M.
Yap, A. S.
Title Myosin 2 is a key Rho kinase target necessary for the local concentration of E-cadherin at cell-cell contacts
Journal name Molecular Biology of The Cell   Check publisher's open access policy
ISSN 1059-1524
1939-4586
Publication date 2005-10
Sub-type Article (original research)
DOI 10.1091/mbc.E05-04-0330
Open Access Status File (Publisher version)
Volume 16
Issue 10
Start page 4531
End page 4542
Total pages 12
Place of publication Bethesda, MD, U.S.A.
Publisher American Society for Cell Biology
Collection year 2005
Language eng
Subject C1
270105 Cellular Interactions (incl. Adhesion, Matrix, Cell Wall)
780105 Biological sciences
0601 Biochemistry and Cell Biology
Formatted abstract
Classical cadherins accumulate at cell-cell contacts as a characteristic response to productive adhesive ligation. Such local accumulation of cadherins is a developmentally regulated process that supports cell adhesiveness and cell-cell cohesion. Yet the molecular effectors responsible for cadherin accumulation remain incompletely understood. We now report that Myosin 2 is critical for cells to concentrate E-cadherin at cell-cell contacts. Myosin 2 is found at cadherin-based cell-cell contacts and its recruitment requires E-cadherin activity. Indeed, both Myosin 2 recruitment and its activation were stimulated by E-cadherin homophilic ligation alone. Inhibition of Myosin 2 activity by blebbistatin or ML-7 rapidly impaired the ability of cells to concentrate E-cadherin at adhesive contacts, accompanied by decreased cadherin-based cell adhesiveness. The total surface expression of cadherins was unaffected, suggesting that Myosin 2 principally regulates the regional distribution of cadherins at the cell surface. The recruitment of Myosin 2 to cadherin contacts, and its activation, required Rho kinase; furthermore, inhibition of Rho kinase signaling effectively phenocopied the effects of Myosin 2 inhibition. We propose that Myosin 2 is a key effector of Rho-Rho kinase signaling that regulates cell-cell adhesion by determining the ability of cells to concentrate cadherins at contacts in response to homophilic ligation.
© 2005 by The American Society for Cell Biology
Keyword Cell Biology
Adhesive contacts
N-cadherin
Actin cytoskeleton
Family GTPases
II inhibitor
C-cadherin
Expression
Rac
Mechanisms
Light
Q-Index Code C1

 
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Created: Wed, 15 Aug 2007, 05:53:11 EST