Simultaneous liquid chromatographic determination of doxorubicin and its major metabolite doxorubicinol in parrot plasma

Gilbert, C. M., McGeary, R. P., Filippich, L. J., Norris, R. L. G. and Charles, B. G. (2005) Simultaneous liquid chromatographic determination of doxorubicin and its major metabolite doxorubicinol in parrot plasma. Journal of Chromatography B-analytical Technologies In The Biomedical And Life Sciences, 826 1-2: 273-276. doi:10.1016/j.jchromb.2005.08.017


Author Gilbert, C. M.
McGeary, R. P.
Filippich, L. J.
Norris, R. L. G.
Charles, B. G.
Title Simultaneous liquid chromatographic determination of doxorubicin and its major metabolite doxorubicinol in parrot plasma
Journal name Journal of Chromatography B-analytical Technologies In The Biomedical And Life Sciences   Check publisher's open access policy
ISSN 1570-0232
Publication date 2005
Sub-type Article (original research)
DOI 10.1016/j.jchromb.2005.08.017
Volume 826
Issue 1-2
Start page 273
End page 276
Total pages 4
Editor G. Hopfgartner
H.T. Karnes
R. Bischoff
Place of publication Amsterdam
Publisher Elsevier Bv
Collection year 2005
Language eng
Subject C1
320501 Pharmaceutical Sciences and Pharmacy
730108 Cancer and related disorders
300512 Pharmacology
780105 Biological sciences
Abstract A new microscale method is reported for the determination of doxorubicin and its active metabolite, doxorubicinol, in parrot plasma. Sample workup involved acetonitrile protein precipitation, ethyl acetate extraction, followed by back extraction into HCl. Separations were achieved on a phenyl-hexyl column at 30 degrees C using acetonitrile (17%, v/v) in 0.01 M orthophosphoric acid (83%, v/v) delivered via a linear flow program. Fluorometric detection wavelengths were 235 nm (excitation) and 550 nm (emission). Calibration plots were linear (1 2 > 0.999), and recoveries were 71-87% from 20 to 400 ng/mL. Assay imprecision was <= 8.5% and inaccuracy was <= 6.3%. The limit of quantification was 25 ng/mL. (c) 2005 Elsevier B.V. All rights reserved.
Keyword Doxorubicin
Parrot
Plasma
Biochemical Research Methods
Chemistry, Analytical
Doxorubicinol
Liquid Chromatography
Hplc Method
Pharmacokinetics
Assay
Q-Index Code C1

 
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Created: Wed, 15 Aug 2007, 05:51:26 EST