Genetic typing of classical swine fever viruses from Lao PDR by analysis of the 5' non-coding region

Blacksell, Stuart D., Khounsy, Syseng, Boyle, David B., Gleeson, Laurence J., Westbury, Harvey A. and Mackenzie, John S. (2005) Genetic typing of classical swine fever viruses from Lao PDR by analysis of the 5' non-coding region. Virus Genes, 31 3: 349-355. doi:10.1007/s11262-005-3253-0


Author Blacksell, Stuart D.
Khounsy, Syseng
Boyle, David B.
Gleeson, Laurence J.
Westbury, Harvey A.
Mackenzie, John S.
Title Genetic typing of classical swine fever viruses from Lao PDR by analysis of the 5' non-coding region
Formatted title
Genetic typing of classical swine fever viruses from Lao PDR by analysis of the 5′ non-coding region
Journal name Virus Genes   Check publisher's open access policy
ISSN 0920-8569
1572-994X
Publication date 2005-12
Sub-type Article (original research)
DOI 10.1007/s11262-005-3253-0
Volume 31
Issue 3
Start page 349
End page 355
Total pages 7
Place of publication Boston, MA, U.S.A.
Publisher Kluwer Academic
Collection year 2005
Language eng
Subject C1
270303 Virology
780105 Biological sciences
0605 Microbiology
1108 Medical Microbiology
Formatted abstract
The 5′ non-coding region (5′-NCR) of 27 classical swine fever virus (CSFV) isolates from Lao People’s Democratic Republic (Lao PDR) during 1997 and 1999 were amplified by RT-PCR. A 150-bp region of the 5′-NCR amplicons was analysed and compared with reference CSFV of European and Asian origin and a phylogenetic dendrogram constructed. Following analysis, all viruses were determined to belong to genogroup 2. Viruses from Lao PDR grouped on a geographical basis with the majority of northern/central isolates falling into subgroup 2.1 and southern/central isolates falling into subgroup 2.2. These results concur with previous studies of CSF viruses from Lao PDR, although this study recognized the first occurrence of subgroup 2.1 in southern Lao PDR.
© 2005 Springer Science+Business Media, Inc.
Keyword Asia
Classical swine fever virus
Genetic typing
Lao PDR
5′ non-coding region
Pig
Molecular epidemiology
Strains
Pestiviruses
PCR
Q-Index Code C1

 
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Created: Wed, 15 Aug 2007, 05:49:25 EST