A soil-based microbial biofilm exposed to 2,4-D: bacterial community development and establishment of conjugative plasmid pJP4

Aspray, Thomas J., Hansen, Susses K. and Burns, Richard G. (2005) A soil-based microbial biofilm exposed to 2,4-D: bacterial community development and establishment of conjugative plasmid pJP4. Fems Microbiology Ecology, 54 2: 317-327. doi:10.1016/j.femsec.2005.04.007


Author Aspray, Thomas J.
Hansen, Susses K.
Burns, Richard G.
Title A soil-based microbial biofilm exposed to 2,4-D: bacterial community development and establishment of conjugative plasmid pJP4
Journal name Fems Microbiology Ecology   Check publisher's open access policy
ISSN 0168-6496
1574-6941
Publication date 2005-10
Sub-type Article (original research)
DOI 10.1016/j.femsec.2005.04.007
Volume 54
Issue 2
Start page 317
End page 327
Total pages 11
Place of publication Oxford, United Kingdom
Publisher Wiley-Blackwell Publishing
Collection year 2005
Language eng
Subject C1
270307 Microbial Ecology
770502 Land and water management
Abstract A soil suspension was used as a source to initiate the development of microbial communities in flow cells irrigated with 2,4-dichlorophenoxyacetic acid (2,4-D) (25 mu g ml(-1)). Culturable bacterial members of the community were identified by 16S rRNA gene sequencing and found to be members of the genera Pseudomonas, Burkholderia, Collimonas and Rhodococcus. A 2,4-D degrading donor strain, Pseudomonas putida SM 1443 (pJP4::gfp), was inoculated into flow cell chambers containing 2-day old biofilm communities. Transfer of pJP4::gfp from the donor to the bacterial community was detectable as GFP fluorescing cells and images were captured using confocal scanning laser microscopy (GFP fluorescence was repressed in the donor due to the presence of a chromosomally located lacl(q) repressor gene). Approximately 5-10 transconjugant microcolonies, 20-40 mu m in diameter, could be seen to develop in each chamber. A 2,4-D degrading transconjugant strain was isolated from the flow cell system belonging to the genus Burkholderia.
Keyword Green fluorescent protein
2
4-d
Horizontal plasmid transfer
Biofilms
Pjp4
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

 
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Created: Wed, 15 Aug 2007, 05:47:13 EST