The effects of intra-amniotic injection of periodontopathic lipopolysaccharides in sheep

Newnham, John P., Shub, Alexis, Jobe, Alan H., Bird, Philip S., Ikegami, Machiko, Nitsos, Ilias and Moss, Timothy J. M. (2005) The effects of intra-amniotic injection of periodontopathic lipopolysaccharides in sheep. American Journal of Obstetrics And Gynecology, 193 2: 313-321. doi:10.1016/j.ajog.2005.03.065


Author Newnham, John P.
Shub, Alexis
Jobe, Alan H.
Bird, Philip S.
Ikegami, Machiko
Nitsos, Ilias
Moss, Timothy J. M.
Title The effects of intra-amniotic injection of periodontopathic lipopolysaccharides in sheep
Journal name American Journal of Obstetrics And Gynecology   Check publisher's open access policy
ISSN 0002-9378
Publication date 2005-01-01
Sub-type Article (original research)
DOI 10.1016/j.ajog.2005.03.065
Volume 193
Issue 2
Start page 313
End page 321
Total pages 9
Editor G. P. Zupan
T. J. Garite
E. J. Quilligan
M. H. Kim
Place of publication USA
Publisher Mosby Inc
Collection year 2005
Language eng
Subject C1
320899 Dentistry not elsewhere classified
730112 Oro-dental and disorders
Abstract Objective: Periodontal disease may cause several complications of pregnancy, including fetal death. The purpose of this study was to investigate in sheep the effects of the intra-amniotic injection of lipopolysaccharide from 3 periodontopathic organisms and to compare these effects with those resulting from similar injection of Escherichia coli lipopolysaccharide. The outcomes that were studied included the rates of fetal death and the features of inflammation and lung maturation in survivors. Study design: At 118 days of pregnancy, ewes that were bearing single fetuses were allocated at random to receive intra-amniotic injections of saline solution (n = 13 fetuses), or lipopolysaccharide from Porphyromonas gingivalis (in doses from 0.1 to 10 tug [n = 22 fetuses]), Actinobacillus actinomycetemcomitans (10 mg [n = 6 fetuses]; 1 mg [n = 6 fetuses]), Fusobacterium nucleation (10 mg [n = 6 fetuses]) or Escherichia coli (10 mg [n = 14 fetuses]; 1 mg [n = 7 fetuses]). Surviving fetuses were delivered abdominally at 125 days of gestation (term, 150 days). Results: When compared with Escherichia coli lipopolysaccharide at similar dosages, periodontopathic lipopolysaccharides had high rates of fetal lethality. Only 6 of 22 fetuses that were exposed to intra-amniotic Porphyromonas gingivalis lipopolysaccharide survived doses of 0.1 to 10 mg, and only 3 of 6 fetuses survived 10-mg Actinobacillus actinomycetemcomitans lipopolysaccharide. Escherichia coli lipopolysaccharide did not cause fetal loss when given at doses of 10 mg (n = 14 fetuses) or l mg (n = 7 fetuses). Fetuses that survived exposure to these lipopolysaccharides showed features of inflammation in amniotic fluid and cord blood at birth and enhanced lung maturation. Conclusion: Lipopolysaccharides from these 3 periodontopathic organisms have much higher rates of fetal lethality than Escherichia coli lipopolysaccharide but can cause similar intrauterine inflammatory responses and improvements in lung volumes in survivors. Sources of inflammation that are distant from the uterus may underlie a proportion of unexplained stillbirth and other complications of pregnancy. (c) 2005 Mosby, Inc. All rights reserved.
Keyword Obstetrics & Gynecology
Periodontopathic
Lipopolysaccharide
Sheep
Porphyromonas-gingivalis Infection
Low-birth-weight
Preterm Birth
Pregnant Mice
Endotoxin
Activation
Disease
Association
Receptors
Responses
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2006 Higher Education Research Data Collection
School of Dentistry Publications
 
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Citation counts: TR Web of Science Citation Count  Cited 28 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 31 times in Scopus Article | Citations
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Created: Wed, 15 Aug 2007, 15:41:18 EST