Reduced hepatic extraction of palmitate in steatosis correlated to lower level of liver fatty acid binding protein

Hung, Daniel Y., Siebert, Gerhard A., Chang, Ping, Burczynski, Frank J. and Roberts, Michael S. (2005) Reduced hepatic extraction of palmitate in steatosis correlated to lower level of liver fatty acid binding protein. American Journal of Physiology-gastrointestinal And Liver Physiology, 288 1: G93-G100. doi:10.1152/ajpgi.00196.2004


Author Hung, Daniel Y.
Siebert, Gerhard A.
Chang, Ping
Burczynski, Frank J.
Roberts, Michael S.
Title Reduced hepatic extraction of palmitate in steatosis correlated to lower level of liver fatty acid binding protein
Journal name American Journal of Physiology-gastrointestinal And Liver Physiology   Check publisher's open access policy
ISSN 0193-1857
Publication date 2005-01-01
Sub-type Article (original research)
DOI 10.1152/ajpgi.00196.2004
Volume 288
Issue 1
Start page G93
End page G100
Total pages 8
Editor M. Montrose
B. B. Rauner
Place of publication USA
Publisher American Physiological Society
Collection year 2005
Language eng
Subject C1
320503 Clinical Pharmacology and Therapeutics
730118 Organs, diseases and abnormal conditions not elsewhere classified
Abstract Nonalcoholic fatty liver disease is the most common of all liver diseases. The hepatic disposition [H-3]palmitate and its low-molecular-weight metabolites in perfused normal and steatotic rat liver were studied using the multiple indicator dilution technique and a physiologically based slow diffusion/bound pharmacokinetic model. The steatotic rat model was established by administration of 17alpha-ethynylestradiol to female Wistar rats. Serum biochemistry markers and histology of treated and normal animals were assessed and indicated the presence of steatosis in the treatment group. The steatotic group showed a significantly higher alanine aminotransferase-to-aspartate aminotransferase ratio, lower levels of liver fatty acid binding protein and cytochrome P-450, as well as microvesicular steatosis with an enlargement of sinusoidal space. Hepatic extraction for unchanged [H-3]palmitate and production of low-molecular-weight metabolites were found to be significantly decreased in steatotic animals. Pharmacokinetic analysis suggested that the reduced extraction and sequestration for palmitate and its metabolites was mainly attributed to a reduction in liver fatty acid binding protein in steatosis.
Keyword Gastroenterology & Hepatology
Physiology
17 Alpha-ethynylestradiol
Hepatic Palmitate Disposition
Residence Time Distributions
Nonalcoholic Steatohepatitis
Cryptogenic Cirrhosis
Rat
Model
Transport
Disease
Albumin
Chain
Hepatocytes
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
2006 Higher Education Research Data Collection
School of Medicine Publications
 
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