Characterization of heat shock protein-specific T cells in atherosclerosis

Ford, Pauline, Gemmell, Erica, Walker, Philip, West, Malcolm, Cullinan, Mary and Seymour, Gregory (2005) Characterization of heat shock protein-specific T cells in atherosclerosis. Clinical And Diagnostic Laboratory Immunology, 12 2: 259-267. doi:10.1128/CDLI.12.2.259-267.2005


Author Ford, Pauline
Gemmell, Erica
Walker, Philip
West, Malcolm
Cullinan, Mary
Seymour, Gregory
Title Characterization of heat shock protein-specific T cells in atherosclerosis
Journal name Clinical And Diagnostic Laboratory Immunology   Check publisher's open access policy
ISSN 1071-412X
Publication date 2005-02
Sub-type Article (original research)
DOI 10.1128/CDLI.12.2.259-267.2005
Volume 12
Issue 2
Start page 259
End page 267
Total pages 9
Editor S. D. Douglas
Place of publication Washington
Publisher American Society for Microbiology
Collection year 2005
Language eng
Abstract A role for infection and inflammation in atherogenesis is widely accepted. Arterial endothelium has been shown to express heat shock protein 60 (HSP60) and, since human (hHSP60) and bacterial (GroEL) HSP60s are highly conserved, the immune response to bacteria may result in cross-reactivity, leading to endothelial damage and thus contribute to the pathogenesis of atherosclerosis. In this study, GroEL-specific T-cell lines from peripheral blood and GroEL-, hHSP60-, and Porphyromonas gingivalis-specific T-cell lines from atherosclerotic plaques were established and characterized in terms of their cross-reactive proliferative responses, cytokine and chemokine profiles, and T-cell receptor (TCR) V beta expression by flow cytometry. The cross-reactivity of several lines was demonstrated. The cytokine profiles of the artery T-cell lines specific for GroEL, hHSP60, and P. gingivalis demonstrated Th2 phenotype predominance in the CD4 subset and Tc0 phenotype predominance in the CD8 subset. A higher proportion of CD4 cells were positive for interferon-inducible protein 10 and RANTES, with low percentages of cells positive for monocyte chemoattractant protein 1 and macrophage inflammatory protein la, whereas a high percentage of CD8 cells expressed all four chemokines. Finally, there was overexpression of the TCR V beta 5.2 family in all lines. These cytokine, chemokine, and V beta profiles are similar to those demonstrated previously for P. gingivalis-specific lines established from periodontal disease patients. These results support the hypothesis that in some patients cross-reactivity of the immune response to bacterial HSPs, including those of periodontal pathogens, with arterial endothelial cells expressing hHSP60 may explain the apparent association between atherosclerosis and periodontal infection.
Keyword Immunology
Infectious Diseases
Microbiology
Porphyromonas-gingivalis
Periodontitis Patients
Atheromatous Plaques
Immune-responses
Cxc Chemokines
Carotid-artery
Ifn-gamma
Expression
Infection
Cytokine
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
2006 Higher Education Research Data Collection
School of Dentistry Publications
 
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Created: Wed, 15 Aug 2007, 05:32:07 EST