mecA locus diversity in methicillin-resistant Staphylococcus aureus isolates in Brisbane, Australia, and the development of a novel diagnostic procedure for the Western Samoan phage pattern clone

Huygens, Flavia, Stephens, Alex J., Nimmo, Graeme R. and Giffard, Philip M. (2004) mecA locus diversity in methicillin-resistant Staphylococcus aureus isolates in Brisbane, Australia, and the development of a novel diagnostic procedure for the Western Samoan phage pattern clone. Journal of Clinical Microbiology, 42 5: 1947-1955. doi:10.1128/JCM.42.5.1947-1955.2004

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Author Huygens, Flavia
Stephens, Alex J.
Nimmo, Graeme R.
Giffard, Philip M.
Title mecA locus diversity in methicillin-resistant Staphylococcus aureus isolates in Brisbane, Australia, and the development of a novel diagnostic procedure for the Western Samoan phage pattern clone
Journal name Journal of Clinical Microbiology   Check publisher's open access policy
ISSN 0095-1137
1098-660X
Publication date 2004-05-01
Sub-type Article (original research)
DOI 10.1128/JCM.42.5.1947-1955.2004
Open Access Status File (Publisher version)
Volume 42
Issue 5
Start page 1947
End page 1955
Total pages 9
Editor R. C. Tilton
A. B. Orderdonk
Place of publication Washington, DC, United States
Publisher American Society for Microbiology
Collection year 2004
Language eng
Abstract An emerging public health phenomenon is the increasing incidence of methicillin-resistant Staphylococcus aureus (MRSA) infections that are acquired outside of health care facilities. One lineage of community-acquired MRSA (CA-MRSA) is known as the Western Samoan phage pattern (WSPP) clone. The central aim of this study was to develop an efficient genotyping procedure for the identification of WSPP isolates. The approach taken was to make use of the highly variable region downstream of mecA in combination with a single nucleotide polymorphism (SNP) defined by the S. aureus multilocus sequence typing (MLST) database. The premise was that a combinatorial genotyping method that interrogated both a highly variable region and the genomic backbone would deliver a high degree of informative power relative to the number of genetic polymorphisms-interrogated. Thirty-five MRSA isolates were used for this study, and their gene contents and order downstream of mecA were determined. The CA-MRSA isolates were found to contain a truncated mecA downstream region consisting of mecA-HVR-IS431 mec-dcs-Ins117, and a PCR-based method for identifying this structure was developed. The hospital-acquired isolates were found to contain eight different mecA downstream regions, three of which were novel. The Minimum SNPs computer software program was used to mine the S. aureus MLST database, and the arcC 2726 polymorph was identified as 82% discriminatory for ST-30. A real-time PCR assay was developed to interrogate this SNP. We found that the assay for the truncated mecA downstream region in combination with the interrogation of arcC position 272 provided an unambiguous identification of WSPP isolates.
Keyword Microbiology
Cassette Chromosome Mec
Penicillin-binding Protein
Community
Element
Mrsa
Identification
Queensland
Evolution
Spread
Pcr
Q-Index Code C1

 
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Created: Wed, 15 Aug 2007, 15:11:31 EST