Antigenic and genetic typing of Whataroa viruses in Australia

Saleh, S. M., Poidinger, M., MacKenzie, J. S., Broom, A. K., Lindsay, M. D. A., Russell, R. C., Cloonan, M. J., Calisher, C. H., Burgess, G. W. and Hall, R. A. (2004) Antigenic and genetic typing of Whataroa viruses in Australia. American Journal of Tropical Medicine And Hygiene, 71 3: 262-267.

Author Saleh, S. M.
Poidinger, M.
MacKenzie, J. S.
Broom, A. K.
Lindsay, M. D. A.
Russell, R. C.
Cloonan, M. J.
Calisher, C. H.
Burgess, G. W.
Hall, R. A.
Title Antigenic and genetic typing of Whataroa viruses in Australia
Journal name American Journal of Tropical Medicine And Hygiene   Check publisher's open access policy
ISSN 0002-9637
Publication date 2004
Sub-type Article (original research)
Volume 71
Issue 3
Start page 262
End page 267
Total pages 6
Place of publication McLean, USA
Publisher American Society of Tropical Medicine & Hygiene
Collection year 2004
Language eng
Subject C1
270303 Virology
270308 Microbial Systematics, Taxonomy and Phylogeny
780105 Biological sciences
Abstract We recently characterized three novel alphaviruses isolated from mosquitoes captured in New South Wales, Australia. Initial cross-neutralization studies revealed antigenic similarity to the Sindbis virus (SINV)-like Whataroa virus (WHAV), heretofore found only in New Zealand. Nucleotide sequence analysis showed that the WHAV-Iike viruses shared >99% nucleotide sequence similarity with each other, and 96-97% similarity with prototype WHAV. Enzyme-linked immunosorbent assay reactions of a panel of monoclonal antibodies to SINV showed that the novel WHAV-Iike viruses displayed identical binding patterns and were antigenically distinct from all SINV isolates examined. Although these viruses displayed a similar binding pattern to prototype WHAV, three monoclonal antibodies discriminated them from the New Zealand virus. Our results suggest that these novel alphaviruses are antigenic variants of WHAV and represent the first reported isolations of this virus from outside New Zealand. The monoclonal antibodies used in this study will be useful for typing new SINV and SINV-like isolates.
Keyword Public, Environmental & Occupational Health
Tropical Medicine
Complete Nucleotide-sequence
Sindbis Virus
South Westland
Q-Index Code C1

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Created: Wed, 15 Aug 2007, 05:07:00 EST