Group A streptococcal vaccine delivery by immunization with a self-adjuvanting M protein-based lipid core peptide construct

Olive, C., Batzloff, M., Horvath, A., Clair, T., Yarwood, P., Toth, I. and Good, M. F. (2004) Group A streptococcal vaccine delivery by immunization with a self-adjuvanting M protein-based lipid core peptide construct. Indian Journal of Medical Research, 119 Suppl.: 88-94.

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Author Olive, C.
Batzloff, M.
Horvath, A.
Clair, T.
Yarwood, P.
Toth, I.
Good, M. F.
Title Group A streptococcal vaccine delivery by immunization with a self-adjuvanting M protein-based lipid core peptide construct
Journal name Indian Journal of Medical Research   Check publisher's open access policy
ISSN 0971-5916
Publication date 2004
Sub-type Article (original research)
Open Access Status File (Publisher version)
Volume 119
Issue Suppl.
Start page 88
End page 94
Total pages 7
Place of publication New Delhi, India
Publisher Indian Council of Medical Research
Collection year 2004
Language eng
Subject C1
250302 Biological and Medical Chemistry
780105 Biological sciences
11 Medical and Health Sciences
Abstract Background & objectives: To develop a broad strain coverage GAS vaccine, several strategies have been investigated which included multi-epitope approaches as well as targeting the M protein conserved C-region. These approaches, however, have relied on the use of adjuvants that are toxic for human application. The development of safe and effective adjuvants for human use is a key issue in the development of effective vaccines. In this study, we investigated the lipid polylysine core peptide (LCP) system as a self-adjuvanting GAS vaccine delivery approach. Methods: An LCP-GAS construct was synthesised incorporating multiple copies of a protective peptide epitope (J8) from the conserved carboxy terminal C-repeat region of the M protein. B10.BR mice were immunized parenterally with the LCP-J8 construct, with or without conventional adjuvant, prior to the assessment of immunogenicity and the induction of serum opsonic antibodies. Results: Our data demonstrated immunogenicity of LCP-J8 when coadministered in complete Freund's adjuvant (CFA), or administered in the absence of conventional adjuvant. In both cases, immunization led to the induction of high-titre J8 peptide-specific serum IgG antibody responses, and the induction of heterologous opsonic antibodies that did not cross-react with human heart tissue proteins. Interpretation & conclusion: These data indicated the potential of a novel self-adjuvanting LCP vaccine delivery system incorporating a synthetic GAS M protein C-region peptide immunogen in the induction of broadly protective immune responses, and pointed to the potential application of this system in human vaccine development against infectious diseases.
Keyword Immunology
Medicine, General & Internal
Medicine, Research & Experimental
Gas Vaccine Delivery
Lipid Core Peptides
M Protein
Synthetic Peptide Vaccines
B-cell Epitopes
Mucosal Colonization
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
2005 Higher Education Research Data Collection
School of Pharmacy Publications
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Created: Wed, 15 Aug 2007, 05:01:46 EST