Increased duodenal expression of divalent metal transporter 1 and iron-regulated gene 1 in cirrhosis

Stuart, KA, Anderson, GJ, Frazer, DM, Murphy, TL, Powell, LW, Fletcher, LM and Crawford, DH (2004) Increased duodenal expression of divalent metal transporter 1 and iron-regulated gene 1 in cirrhosis. Hepatology, 39 2: 492-499. doi:10.1002/hep.20038

Author Stuart, KA
Anderson, GJ
Frazer, DM
Murphy, TL
Powell, LW
Fletcher, LM
Crawford, DH
Title Increased duodenal expression of divalent metal transporter 1 and iron-regulated gene 1 in cirrhosis
Journal name Hepatology   Check publisher's open access policy
ISSN 0270-9139
Publication date 2004
Sub-type Article (original research)
DOI 10.1002/hep.20038
Volume 39
Issue 2
Start page 492
End page 499
Total pages 8
Editor A. T. Blei
G. Bologna
Place of publication USA
Publisher John Wiley & Sons Inc
Collection year 2004
Language eng
Subject C1
321006 Gastroenterology and Hepatology
730118 Organs, diseases and abnormal conditions not elsewhere classified
Abstract Hepatic hemosiderosis and increased iron absorption are common findings in cirrhosis. It has been proposed that a positive relation exists between intestinal iron absorption and the development of hepatic hemosiderosis. The current study investigated the duodenal expression of the iron transport molecules divalent metal transporter 1 (DMT1 [IRE]), iron-regulated gene 1 (Ireg1 [ferroportin]), hephaestin, and duodenal cytochrome b (Dyctb) in 46 patients with cirrhosis and 20 control subjects. Total RNA samples were extracted from duodenal biopsy samples and the expression of the iron transport genes was assessed by ribonuclease protection assays. Expression of DMT1 and Ireg1 was increased 1.5 to 3-fold in subjects with cirrhosis compared with iron-replete control subjects. The presence of cirrhosis per se and serum ferritin (SF) concentration were independent factors that influenced the expression of DMT1. However, only SF concentration was independently associated with Iregl expression. In cirrhosis, the expression of DMT1 and Iregl was not related to the severity of liver disease or cirrhosis type. There was no correlation between the duodenal expression of DMT1 and Iregl and the degree of hepatic siderosis. In conclusion, the presence of cirrhosis is an independent factor associated with increased expression of DMT1 but not Iregl. The mechanism by which cirrhosis mediates this change in DMT1 expression has yet to be determined. Increased expression of DMT1 may play an important role in the pathogenesis of cirrhosis-associated hepatic iron overload.
Keyword Gastroenterology & Hepatology
Hepatic Iron
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
2005 Higher Education Research Data Collection
School of Medicine Publications
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Citation counts: TR Web of Science Citation Count  Cited 13 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 14 times in Scopus Article | Citations
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Created: Wed, 15 Aug 2007, 04:57:36 EST