Insertion of an E-coli lacZ gene in Acetobacter xylinus for the production of cellulose in whey

Battad-Bernardo, E., McCrindle, S. L., Couperwhite, I. and Neilan, B. A. (2004) Insertion of an E-coli lacZ gene in Acetobacter xylinus for the production of cellulose in whey. Fems Microbiology Letters, 231 2: 253-260. doi:10.1016/S0378-1097(04)00007-2


Author Battad-Bernardo, E.
McCrindle, S. L.
Couperwhite, I.
Neilan, B. A.
Title Insertion of an E-coli lacZ gene in Acetobacter xylinus for the production of cellulose in whey
Journal name Fems Microbiology Letters   Check publisher's open access policy
ISSN 0378-1097
Publication date 2004
Sub-type Article (original research)
DOI 10.1016/S0378-1097(04)00007-2
Volume 231
Issue 2
Start page 253
End page 260
Total pages 8
Place of publication Amsterdam
Publisher Elsevier
Collection year 2004
Language eng
Subject C1
270200 Genetics
780105 Biological sciences
Abstract A mini-Tn10:lacZ: kan was inserted into a wild-type strain of Acetobacter xylinus by random transposon mutagenesis, generating a lactose-utilising and cellulose-producing mutant strain designated ITz3. Antibiotic selection plate assays and Southern hybridisation revealed that the lacZ gene was inserted once into the chromosome of strain ITz3 and was stably maintained in non-selective medium after more than 60 generations. The modified strain had, on the average, a 28-fold increase in cellulose production and a 160-fold increase in beta-galactosidase activity when grown in lactose medium. beta-Galactosidase activity is present in either lactose or sucrose medium indicating that the gene is constitutively expressed. Cellulose and beta-galactosidase production by the modified strain was also evaluated in pure and enriched whey substrates. Utilisation of lactose in whey substrate by ITz3 reached 17 g l(-1) after 4 days incubation. (C) 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.
Keyword Microbiology
Acetobacter
Cellulose
Mini-tn10
Transposon Mutagenesis
Whey
Utilizing Xanthomonas-campestris
Bacterial Cellulose
Phosphotransferase System
Fermentation Substrate
Escherichia-coli
Xanthan Gum
Lactose
Glucose
Construction
Biosynthesis
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2005 Higher Education Research Data Collection
School of Chemistry and Molecular Biosciences
 
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Created: Wed, 15 Aug 2007, 04:55:04 EST